PMID- 8651705
OWN - NLM
STAT- MEDLINE
DCOM- 19960722
LR  - 20190629
IS  - 0003-9861 (Print)
IS  - 0003-9861 (Linking)
VI  - 330
IP  - 1
DP  - 1996 Jun 1
TI  - Differential expression of the two duplicated insecticyanin genes, ins-a and 
      ins-b, in the black mutant of Manduca sexta.
PG  - 65-70
AB  - The black (bl) mutant larvae of Manduca sexta produces no detectable juvenile 
      hormone (JH) during the molt to the 5th instar. To investigate the JH control of 
      expression of the two insecticyanin genes, ins-a and ins-b, developmental changes 
      of the two mRNAs were examined in this mutant. In the epidermis of the freshly 
      ecdysed larvae, the two mRNAs were hardly detectable. Twelve hours after ecdysis, 
      the two mRNAs appeared in low levels and were then gradually increased with ins-a 
      mRNA rising to a plateau from Day 1 to Day 2 morning and ins-b mRNA in Day 2. 
      During these early developmental stages, the epidermal level of the two mRNAs in 
      the bl mutant was substantially lower than that observed in the wild-type larvae. 
      On Day 3 of the 5th instar when the larval epidermis becomes pupally committed, 
      the two mRNAs in both bl mutant and wild-type larvae show a similar differential 
      expression pattern. In the fat body, the level of both ins-a and ins-b mRNAs was 
      higher than that found in the wild-type larvae. Exogenous JH almost restores the 
      level of the two mRNAs in both epidermis and fat body of the Day 0 5th-instar bl 
      mutant larvae to that observed in wild-type animals of the same stage. These 
      results suggest that JH may enhance expression of the two insecticyanin genes in 
      the epidermis but partially repress their expression in the fat body during early 
      development of the wild-type 5th-instar larvae.
FAU - Li, W C
AU  - Li WC
AD  - Department of Zoology, Unversity of Washington, Seattle 98195, USA.
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Arch Biochem Biophys
JT  - Archives of biochemistry and biophysics
JID - 0372430
RN  - 0 (Insect Proteins)
RN  - 0 (Invertebrate Hormones)
RN  - 0 (RNA, Messenger)
RN  - 0 (insecticyanin protein, insect)
SB  - IM
EIN - Arch Biochem Biophys 1997 Feb 1;338(1):128
MH  - Animals
MH  - Gene Expression Regulation, Developmental
MH  - *Genes, Insect
MH  - *Insect Proteins
MH  - Invertebrate Hormones/*biosynthesis/genetics
MH  - Larva
MH  - Manduca/genetics/growth & development/*metabolism
MH  - Multigene Family
MH  - Mutation
MH  - RNA, Messenger/biosynthesis
MH  - Skin/metabolism
MH  - *Transcription, Genetic
EDAT- 1996/06/01 00:00
MHDA- 1996/06/01 00:01
CRDT- 1996/06/01 00:00
PHST- 1996/06/01 00:00 [pubmed]
PHST- 1996/06/01 00:01 [medline]
PHST- 1996/06/01 00:00 [entrez]
AID - S000398619690226X [pii]
AID - 10.1006/abbi.1996.0226 [doi]
PST - ppublish
SO  - Arch Biochem Biophys. 1996 Jun 1;330(1):65-70. doi: 10.1006/abbi.1996.0226.