PMID- 8652379
OWN - NLM
STAT- MEDLINE
DCOM- 19960729
LR  - 20190705
IS  - 0007-1048 (Print)
IS  - 0007-1048 (Linking)
VI  - 93
IP  - 3
DP  - 1996 Jun
TI  - Cytogenetic clonality analysis: typical patterns in myelodysplastic syndrome and 
      acute myeloid leukaemia.
PG  - 594-600
AB  - The cell morphology and karyotype of bone marrow samples from 24 patients with 
      myelodysplastic syndrome (MDS) and acute myeloid leukaemia (AML) were studied 
      simultaneously with a combined technique of May-Grunwald-Giemsa (MGG) staining 
      and fluorescence in situ hybridization (FISH) with chromosome-specific DNA 
      probes. This enabled us to investigate cell lineage involvement in three 
      malignant conditions: MDS (n = 12), leukaemia-transformed MDS (LT-MDS) (n = 5) 
      and de novo AML (n = 7). In MDS we found blasts and often significant proportions 
      of mature granulocytic and erythroid cells to be cytogenetically abnormal. 
      Percentages of granulocytic and erythroid cells with cytogenetic aberrations were 
      generally less than those of blasts. These data support the involvement of a 
      transformed pluripotent stem cell that has retained maturation abilities. In two 
      patients with chronic myelomonocytic leukaemia (CMMoL) the clonal involvement of 
      monocytes was predominant. Results in the five patients with LT-MDS were similar 
      to those in MDS. In the bone marrow of five of the seven de novo AML patients the 
      cytogenetic abnormalities were restricted to the blasts and did not include the 
      more mature granulocytic or erythroid populations. In the other two patients with 
      AML, both with a t(8;21) and a loss of the Y chromosome, high percentages of 
      mature neutrophils were cytogenetically abnormal. These patterns of clonal 
      lineage involvement in MDS, LT-MDS, t(8;21) AML and AML appear typical and may be 
      of clinical use, for example, for distinguishing LT-MDS from de novo AML in newly 
      presenting patients.
FAU - van Lom, K
AU  - van Lom K
AD  - Department of Haematology, University Hospital, Erasmus University Rotterdam, The 
      Netherlands.
FAU - Hagemeijer, A
AU  - Hagemeijer A
FAU - Vandekerckhove, F
AU  - Vandekerckhove F
FAU - Smit, E M
AU  - Smit EM
FAU - Lowenberg, B
AU  - Lowenberg B
LA  - eng
PT  - Journal Article
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
SB  - IM
MH  - Acute Disease
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Bone Marrow/pathology
MH  - Cloning, Molecular
MH  - Erythroblasts/pathology
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Leukemia, Myeloid/genetics/*pathology
MH  - Male
MH  - Middle Aged
MH  - Myelodysplastic Syndromes/genetics/*pathology
EDAT- 1996/06/01 00:00
MHDA- 1996/06/01 00:01
CRDT- 1996/06/01 00:00
PHST- 1996/06/01 00:00 [pubmed]
PHST- 1996/06/01 00:01 [medline]
PHST- 1996/06/01 00:00 [entrez]
AID - 10.1046/j.1365-2141.1996.d01-1692.x [doi]
PST - ppublish
SO  - Br J Haematol. 1996 Jun;93(3):594-600. doi: 10.1046/j.1365-2141.1996.d01-1692.x.