PMID- 8653677
OWN - NLM
STAT- MEDLINE
DCOM- 19960730
LR  - 20131121
IS  - 0008-5472 (Print)
IS  - 0008-5472 (Linking)
VI  - 56
IP  - 11
DP  - 1996 Jun 1
TI  - Glucose catabolism in cancer cells: amplification of the gene encoding type II 
      hexokinase.
PG  - 2468-71
AB  - Hexokinase type II is highly overexpressed in many cancer cells, where it plays a 
      pivotal role in the high glycolytic phenotype. Here we demonstrate by Southern 
      blot analysis and fluorescence in situ hybridization (FISH) that in the rapidly 
      growing rat AS-30D hepatoma cell line, enhanced hexokinase activity is associated 
      with at least a 5-fold amplification of the type II gene relative to normal 
      hepatocytes. This amplification is located chromosomally, extends to the whole 
      gene, and most likely occurs at the site of the resident gene. No rearrangement 
      of the gene could be detected. Therefore, overexpression of hexokinase type II in 
      AS-30D hepatoma cells may be based, at least in part, on a stable gene 
      amplification. This is the first report describing the amplification of a 
      hexokinase gene in a tumor cell line expressing the high glycolytic phenotype.
FAU - Rempel, A
AU  - Rempel A
AD  - Department of Biological Chemistry, The Johns Hopkins University, School of 
      Medicine, Baltimore, Maryland 21205-2185, USA.
FAU - Mathupala, S P
AU  - Mathupala SP
FAU - Griffin, C A
AU  - Griffin CA
FAU - Hawkins, A L
AU  - Hawkins AL
FAU - Pedersen, P L
AU  - Pedersen PL
LA  - eng
GR  - 2P30-CA06972/CA/NCI NIH HHS/United States
GR  - CA 32742/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cancer Res
JT  - Cancer research
JID - 2984705R
RN  - 0 (DNA, Neoplasm)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - DNA, Neoplasm/genetics
MH  - Female
MH  - Gene Expression Regulation, Enzymologic
MH  - Gene Expression Regulation, Neoplastic
MH  - Glucose/*metabolism
MH  - Hexokinase/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Polymorphism, Restriction Fragment Length
MH  - RNA, Messenger/genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 1996/06/01 00:00
MHDA- 1996/06/01 00:01
CRDT- 1996/06/01 00:00
PHST- 1996/06/01 00:00 [pubmed]
PHST- 1996/06/01 00:01 [medline]
PHST- 1996/06/01 00:00 [entrez]
PST - ppublish
SO  - Cancer Res. 1996 Jun 1;56(11):2468-71.