PMID- 8654521
OWN - NLM
STAT- MEDLINE
DCOM- 19960731
LR  - 20141120
IS  - 0014-4886 (Print)
IS  - 0014-4886 (Linking)
VI  - 139
IP  - 2
DP  - 1996 Jun
TI  - Traumatized rat striatum produces neurite-promoting and neurotrophic activities 
      in vitro.
PG  - 173-87
AB  - We have previously reported that ciliary neurotrophic factor (CNTF) mRNA is 
      upregulated in the rat striatum following trauma and that its peak is coincident 
      with a peak in the number of GFAP-positive astrocytes. CNTF, or other 
      neurotrophic factors present in the traumatized striatum, may be involved in the 
      dopaminergic fiber sprouting seen following cavitation or graft implantation in 
      animal models of Parkinson's disease. This study was undertaken in order to 
      further characterize the neurotrophic activity present following trauma through 
      the use of bioassays. Adult rats underwent stereotaxic biopsy of the right 
      striatum, and gelatin sponge [gelfoam (GF)] was placed in the resultant cavity. 
      GF was collected from 1 to 30 days following trauma and homogenized. GF extracts 
      (with equal protein concentrations) were assayed using dorsal root ganglion (DRG) 
      explants, dissociated ciliary ganglia (CG), and human dopaminergic neuroblastoma 
      cell (SH-SY5Y) cultures. The GF extracts had significant neurite-promoting 
      activity (NPA) for DRG, CG, and SH-SY5Y cells, with the maximum effect seen 7 
      days after trauma. NPA was not blocked by anti-nerve growth factor (NGF) Ab, but 
      anti-brain-derived neurotrophic factor (BDNF) Ab significantly blocked the 
      activity for DRG. The GF extracts protected the SH-SY5Y cells from the 
      neurotoxins 6-OHDA and MPP+, as did NGF and BDNF. This neuroprotective effect of 
      GF was not blocked by anti-NGF Ab. This study suggests that the neurotrophic 
      activity in GF extracts has CNTF-like and BDNF-like components as well as 
      another, undefined component.
FAU - Asada, H
AU  - Asada H
AD  - Department of Neurosurgery, State University of New York at buffalo, New York 
      14209, USA.
FAU - Kaseloo, P A
AU  - Kaseloo PA
FAU - Lis, A
AU  - Lis A
FAU - Petti, D M
AU  - Petti DM
FAU - Plunkett, R J
AU  - Plunkett RJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Exp Neurol
JT  - Experimental neurology
JID - 0370712
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Ciliary Neurotrophic Factor)
RN  - 0 (Cytokines)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor
MH  - Cells, Cultured
MH  - Chick Embryo
MH  - Ciliary Neurotrophic Factor
MH  - Corpus Striatum/*drug effects
MH  - Cytokines/immunology
MH  - Dose-Response Relationship, Drug
MH  - Gelatin Sponge, Absorbable/pharmacology
MH  - Humans
MH  - In Vitro Techniques
MH  - Male
MH  - Nerve Growth Factors/pharmacology
MH  - Nerve Tissue Proteins/*pharmacology
MH  - Neurites/*drug effects
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 1996/06/01 00:00
MHDA- 1996/06/01 00:01
CRDT- 1996/06/01 00:00
PHST- 1996/06/01 00:00 [pubmed]
PHST- 1996/06/01 00:01 [medline]
PHST- 1996/06/01 00:00 [entrez]
AID - S0014-4886(96)90092-8 [pii]
AID - 10.1006/exnr.1996.0092 [doi]
PST - ppublish
SO  - Exp Neurol. 1996 Jun;139(2):173-87. doi: 10.1006/exnr.1996.0092.