PMID- 8654909
OWN - NLM
STAT- MEDLINE
DCOM- 19960731
LR  - 20220309
IS  - 0831-2796 (Print)
IS  - 0831-2796 (Linking)
VI  - 38
IP  - 6
DP  - 1995 Dec
TI  - Repeated DNA sequences isolated by microdissection. I. Karyotyping of barley 
      (Hordeum vulgare L.).
PG  - 1082-90
AB  - We report on microdissection, cloning and sequence, and Southern and fluorescence 
      in situ hybridization (FISH) analysis of one moderately and one highly amplified 
      repetitive DNA element, pHvMWG2314 and pHvMWG2315, respectively, isolated from 
      barley (Hordeum vulgare L.) chromosome arm 3HL. The pHvMWG2315 sequence 
      hybridizes to all 14 telomeric or subtelomeric regions of the barley chromosomes 
      as determined by FISH. The 50 different hybridization sites that include 
      intercalary signals allow the discrimination of all 14 chromosome arms and the 
      construction of a kariotype of barley. The tandemly repeated subtelomeric element 
      of 331 bp exists in all Triticeae species tested (H. vulgare, Agropyron 
      elongatum, Secale cereale, Triticum tauschii, T. turgidum, and T. aestivum). It 
      is AT rich (66%), exibits 84% sequence homology to subfragments of the D genome 
       inverted question markspecific inverted question mark 1-kb element pAs1 of T. tauscii and 75% homology to interspersed 
      genome-specific DNA sequence pHcKB6 from H. chilence. The repetitive sequence 
      pHvMWG2314 is moderately amplified in barley and highly amplified in hexaploid 
      wheat. The in situ experiments revealed no distinct signals on barley 
      chromosomes, indicating a dispersed character for the sequence. The significance 
      of the results for the identification of chromosomes and chromosome aberrations 
      in FISH experiments are discussed.
FAU - Busch, W
AU  - Busch W
AD  - Botanisches Institut, Ludwig-Maximilians-Universitat Munchen, Germany.
FAU - Martin, R
AU  - Martin R
FAU - Herrmann, R G
AU  - Herrmann RG
FAU - Hohmann, U
AU  - Hohmann U
LA  - eng
SI  - GENBANK/D30736
SI  - GENBANK/X85383
SI  - GENBANK/X85384
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Genome
JT  - Genome
JID - 8704544
RN  - 0 (DNA, Plant)
SB  - IM
MH  - Base Composition
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA, Plant/*genetics/isolation & purification
MH  - *Genetic Techniques
MH  - Hordeum/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping/*methods
MH  - Molecular Sequence Data
MH  - Repetitive Sequences, Nucleic Acid/*genetics
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Nucleic Acid
MH  - Triticum/genetics
EDAT- 1995/12/01 00:00
MHDA- 1995/12/01 00:01
CRDT- 1995/12/01 00:00
PHST- 1995/12/01 00:00 [pubmed]
PHST- 1995/12/01 00:01 [medline]
PHST- 1995/12/01 00:00 [entrez]
AID - 10.1139/g95-144 [doi]
PST - ppublish
SO  - Genome. 1995 Dec;38(6):1082-90. doi: 10.1139/g95-144.