PMID- 8655961
OWN - NLM
STAT- MEDLINE
DCOM- 19960731
LR  - 20190817
IS  - 0168-8278 (Print)
IS  - 0168-8278 (Linking)
VI  - 23
IP  - 4
DP  - 1995 Oct
TI  - Regulation of ferritin expression by alcohol in a human hepatoblastoma cell line 
      and in rat hepatocyte cultures.
PG  - 431-9
AB  - Serum ferritin increases in chronic alcoholism, without clear explanation. We 
      have previously shown that alcohol increases ferritin levels in a human 
      hepatoblastoma cell line (HepG2). The aims of the present work were: 1) To extend 
      our results in normal rat hepatocyte cultures, and 2) To determine the mechanism 
      by which alcohol enhances ferritin levels. In HepG2 cells, high alcohol 
      concentrations (300 mM) during long exposure (4 days) increased the synthesis of 
      H and L ferritin subunits, in association with increased levels of ferritin 
      mRNAs. In rat hepatocyte cultures, the synthesis of L ferritin increased after 24 
      h of exposure to lower alcohol concentrations (10 mM); alcohol had no effect on 
      ferritin mRNAs levels. In both cell types, the alcohol effect was not related to 
      an increase in iron intracellular incorporation. In HepG2 cells, desferrioxamine 
      (Df), a potent iron chelator, abolished ferritin synthesis in the presence or 
      absence of alcohol, and abolished the alcohol induction of ferritin mRNAs. In rat 
      hepatocytes, Df decreased ferritin synthesis to a similar level in the presence 
      or absence of alcohol. Alcohol increased ferritin synthesis differently in HepG2 
      cells and in normal rat hepatocyte cultures. In the latter case, the alcohol 
      effect was observed at low concentration. Despite a striking inhibiting effect of 
      Df on ferritin synthesis, in both cellular models a mechanism accounting for 
      increased ferritin synthesis independently of iron is suggested. Globally, these 
      data strongly suggest that hyperferritinemia in chronic alcoholism could be 
      related to the induction of ferritin by alcohol.
FAU - Moirand, R
AU  - Moirand R
AD  - INSERM U 49, Unite de Recherches Hepatologiques, Hopital Pontchaillou, Rennes, 
      France.
FAU - Kerdavid, F
AU  - Kerdavid F
FAU - Loreal, O
AU  - Loreal O
FAU - Hubert, N
AU  - Hubert N
FAU - Leroyer, P
AU  - Leroyer P
FAU - Brissot, P
AU  - Brissot P
FAU - Lescoat, G
AU  - Lescoat G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Hepatol
JT  - Journal of hepatology
JID - 8503886
RN  - 0 (RNA, Messenger)
RN  - 0 (Transferrin)
RN  - 3K9958V90M (Ethanol)
RN  - 9007-73-2 (Ferritins)
RN  - E1UOL152H7 (Iron)
SB  - IM
MH  - Animals
MH  - Blotting, Northern
MH  - Ethanol/*pharmacology
MH  - Ferritins/*biosynthesis/drug effects
MH  - Hepatoblastoma/etiology/*metabolism/pathology
MH  - Humans
MH  - Iron/metabolism
MH  - Liver Neoplasms/etiology/*metabolism/pathology
MH  - Male
MH  - RNA, Messenger/drug effects/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Transferrin/biosynthesis/drug effects
MH  - Tumor Cells, Cultured
EDAT- 1995/10/01 00:00
MHDA- 1995/10/01 00:01
CRDT- 1995/10/01 00:00
PHST- 1995/10/01 00:00 [pubmed]
PHST- 1995/10/01 00:01 [medline]
PHST- 1995/10/01 00:00 [entrez]
AID - 0168-8278(95)80202-9 [pii]
AID - 10.1016/0168-8278(95)80202-9 [doi]
PST - ppublish
SO  - J Hepatol. 1995 Oct;23(4):431-9. doi: 10.1016/0168-8278(95)80202-9.