PMID- 8656025
OWN - NLM
STAT- MEDLINE
DCOM- 19960730
LR  - 20200304
IS  - 0169-4197 (Print)
IS  - 0169-4197 (Linking)
VI  - 19
IP  - 1
DP  - 1996 Feb
TI  - Pancreatic adenocarcinoma cell line, MDAPanc-28, with features of both acinar and 
      ductal cells.
PG  - 31-8
AB  - CONCLUSION: We established a new human pancreatic adenocarcinoma cell line, 
      MDAPanc-28. Studies on this new line indicate that it expressed both acinar and 
      ductal gene products suggesting that the patterns of gene expression in the 
      pancreatic adenocarcinoma from which this cell line arose have features similar 
      to those of the protodifferentiated cells hypothesized by Rutter and his 
      colleagues for the developing pancreas (1,2). BACKGROUND: The cell line arose 
      from a tumor that, like most pancreatic adenocarcinomas, was ductal on the basis 
      of its histological appearance. METHODS: Once the cell line was established in 
      culture, they were subjected to cytogenetic analysis and tested for their ability 
      to grow in nude mice. RNA from the cells was analyzed by Northern blot analysis 
      and PCR of reverse transcribed cDNA for the expression of both acinar and duct 
      cell gene products. DNA was analyzed for the presence of mutated K-ras at codon 
      12. RESULTS: The cell line expressed trypsin and ribonuclease RNA, which are 
      considered to be acinar cell markers, and carbonic anhydrase II (CAII), which is 
      considered to be a duct-cell markers. The histological appearance of xenografts 
      in nude mice was similar to that of the tumor from which the cell line was 
      established. The chromosome number varied between 46 and 60.
FAU - Frazier, M L
AU  - Frazier ML
AD  - Department of Gastrointestinal Oncology and Digestive Diseases, University of 
      Texas, M. D. Anderson Cancer Center, Houston 77025, USA.
FAU - Fernandez, E
AU  - Fernandez E
FAU - de Llorens, R
AU  - de Llorens R
FAU - Brown, N M
AU  - Brown NM
FAU - Pathak, S
AU  - Pathak S
FAU - Cleary, K R
AU  - Cleary KR
FAU - Abbruzzese, J L
AU  - Abbruzzese JL
FAU - Berry, K
AU  - Berry K
FAU - Olive, M
AU  - Olive M
FAU - Le Maistre, A
AU  - Le Maistre A
FAU - Evans, D B
AU  - Evans DB
LA  - eng
GR  - CA16672/CA/NCI NIH HHS/United States
GR  - R01-CA46687/CA/NCI NIH HHS/United States
GR  - R01-CA49667/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Int J Pancreatol
JT  - International journal of pancreatology : official journal of the International 
      Association of Pancreatology
JID - 8703511
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (RNA, Neoplasm)
RN  - EC 3.1.- (Ribonucleases)
RN  - EC 3.4.21.4 (Trypsin)
RN  - EC 4.2.1.1 (Carbonic Anhydrases)
SB  - IM
MH  - Adenocarcinoma/genetics/metabolism/*pathology
MH  - Aged
MH  - Animals
MH  - Base Sequence
MH  - Biomarkers, Tumor/biosynthesis
MH  - Blotting, Northern
MH  - Carbonic Anhydrases/biosynthesis
MH  - Female
MH  - Genes, ras/genetics
MH  - Humans
MH  - Mice
MH  - Mice, Nude
MH  - Molecular Sequence Data
MH  - Pancreatic Neoplasms/genetics/metabolism/*pathology
MH  - Point Mutation
MH  - Polymerase Chain Reaction
MH  - RNA, Neoplasm/analysis
MH  - Ribonucleases/biosynthesis
MH  - Transplantation, Heterologous
MH  - Trypsin/biosynthesis
MH  - Tumor Cells, Cultured/*pathology
EDAT- 1996/02/01 00:00
MHDA- 1996/02/01 00:01
CRDT- 1996/02/01 00:00
PHST- 1996/02/01 00:00 [pubmed]
PHST- 1996/02/01 00:01 [medline]
PHST- 1996/02/01 00:00 [entrez]
AID - 10.1007/BF02788373 [doi]
PST - ppublish
SO  - Int J Pancreatol. 1996 Feb;19(1):31-8. doi: 10.1007/BF02788373.