PMID- 8656050
OWN - NLM
STAT- MEDLINE
DCOM- 19960801
LR  - 20190516
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 59
IP  - 5
DP  - 1996 May
TI  - Integrin-mediated epithelial-T cell interaction enhances nitric oxide production 
      and increased intracellular inhibition of Chlamydia.
PG  - 656-62
AB  - T cell-mediated immunity against Chlamydia in mice is mediated at least in part 
      by T cell-derived interferon-gamma (IFN-gamma) induction of the nitric oxide 
      synthase (iNOS) system in infected epithelial cells. Although IFN-gamma alone 
      could stimulate nitric oxide (NO) production from epithelial cells and inhibit 
      the intracellular growth of Chlamydia, the effectiveness was less than when 
      infected epithelial cells were co-cultured with IFN-gamma-producing T cell 
      clones. In co-cultures containing T cells and infected epithelial cells, 
      additional NO produced by activated T cells could augment chlamydial killing; 
      however, T cell-derived NO was insufficient to account for the total NO present 
      in the co-culture and therefore could not explain the dramatic increase in 
      chlamydial inhibition under those conditions. To determine whether direct 
      cell-to-cell interaction involving adhesion molecules was involved in increased 
      NO induction, the ability of neutralizing monoclonal antibodies directed against 
      intercellular adhesion molecule type 1 (ICAM-1) and leukocyte function antigen-1 
      (LFA-1) to suppress NO production and lower intracellular chlamydial inhibition 
      was investigated. It was found that monoclonal antibodies against ICAM-1/LFA-1 
      could significantly reduce the capacity of a protective CD4+ type 1 (Thl) clone 
      (clone 2.14-0) to inhibit the intracellular growth of the C. trachomatis agent of 
      mouse pneumonitis (MoPn). The suppression of the anti-chlamydial action of the 
      clone by antibodies correlated with approximately 50% decrease in NO production. 
      Also, paraformaldehyde-fixed clone 2.14-0 could enhance NO induction and 
      chlamydial inhibition mediated by IFN-gamma, and this effect could be reversed by 
      anti-ICAM-1/LFA-1 antibodies. The results indicated that epithelial-T cell 
      interaction via adhesion molecules enhances NO production and increased 
      chlamydial inhibition by IFN-gamma-secreting T cells.
FAU - Igietseme, J U
AU  - Igietseme JU
AD  - Department of Microbiology and Immunology, University of Arkansas for Medical 
      Sciences, Little Rock 72205, USA.
FAU - Uriri, I M
AU  - Uriri IM
FAU - Hawkins, R
AU  - Hawkins R
FAU - Rank, R G
AU  - Rank RG
LA  - eng
GR  - AI26328/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Integrins)
RN  - 0 (Lymphocyte Function-Associated Antigen-1)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Cell Communication
MH  - Cells, Cultured
MH  - Chlamydia/*immunology
MH  - Coculture Techniques
MH  - Epithelial Cells
MH  - Female
MH  - Integrins/*physiology
MH  - Interferon-gamma/pharmacology
MH  - Lymphocyte Function-Associated Antigen-1/physiology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Nitric Oxide/*biosynthesis
MH  - T-Lymphocytes/*physiology
EDAT- 1996/05/01 00:00
MHDA- 1996/05/01 00:01
CRDT- 1996/05/01 00:00
PHST- 1996/05/01 00:00 [pubmed]
PHST- 1996/05/01 00:01 [medline]
PHST- 1996/05/01 00:00 [entrez]
AID - 10.1002/jlb.59.5.656 [doi]
PST - ppublish
SO  - J Leukoc Biol. 1996 May;59(5):656-62. doi: 10.1002/jlb.59.5.656.