PMID- 8656058
OWN - NLM
STAT- MEDLINE
DCOM- 19960801
LR  - 20190516
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 59
IP  - 5
DP  - 1996 May
TI  - IFN-alpha-mediated suppression of low-affinity FC(epsilon) receptors on Peyer's 
      patch lymphocytes and augmentation of soluble CD23: implications for IgE 
      responses.
PG  - 725-7
AB  - The ability of interleukin (IL)-6 or interferon-alpha (IFN-alpha) to regulate 
      expression of low-affinity Fc(epsilon) receptor (CD23) and serum levels of CD23 
      was studied in benzylpenicilloyl-keyhole limpet hemocyanin-sensitized BALB/c mice 
      at the peak of a hapten-specific immunoglobulin E (IgE) antibody-forming cell 
      (AFC) response. These responses are analogous to those observed in human atopic 
      disease. To induce peak IgE responses, mice were injected intraperitoneally with 
      BPO-KLH (10 micrograms) in aluminum hydroxide gel (alum) on days 0, 21, and 42. 
      On day 44, mice were injected subcutaneously with IL-6 (100-1000 U) or IFN-alpha 
      (1000-10,000 U). On day 46, numbers of CD23+ lymphocytes in Peyer's patches (PP), 
      mesenteric lymph nodes (MLN), and spleen and levels of soluble CD23 in serum were 
      determined (flow microfluorimetry and enzyme-linked immunosorbent assay, 
      confirmed by competition assay). Data are expressed as percent total cells or as 
      optical density at 490 nm. IFN-alpha treatment strongly suppressed (up to 100%) 
      numbers of CD23+ cells exclusively in PP (i.e., numbers of CD23+ cells in MLN and 
      spleen were unchanged) whereas serum levels of soluble CD23 were dramatically 
      increased (60%). IL-6 treatment had no effect on either numbers of CD23+ 
      lymphocytes or on serum levels of soluble CD23. The data suggest that the 
      mechanism(s) by which IFN-alpha, but not IL-6, regulates IgE responses involves 
      suppression of CD23 expression on lymphocytes in PPs and supports a central role 
      for these organs in regulation of IgE responses in vivo.
FAU - Miller, H
AU  - Miller H
AD  - Department of Pathology, State University of New York Health Science Center at 
      Brooklyn, 11203, USA.
FAU - Bluth, M H
AU  - Bluth MH
FAU - Chice, S M
AU  - Chice SM
FAU - Durkin, H G
AU  - Durkin HG
FAU - Auci, D L
AU  - Auci DL
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Interferon-alpha)
RN  - 0 (Interleukin-6)
RN  - 0 (Receptors, IgE)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Immunoglobulin E/*biosynthesis
MH  - Interferon-alpha/*pharmacology
MH  - Interleukin-6/pharmacology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Peyer's Patches/*immunology
MH  - Receptors, IgE/analysis/*drug effects
EDAT- 1996/05/01 00:00
MHDA- 1996/05/01 00:01
CRDT- 1996/05/01 00:00
PHST- 1996/05/01 00:00 [pubmed]
PHST- 1996/05/01 00:01 [medline]
PHST- 1996/05/01 00:00 [entrez]
AID - 10.1002/jlb.59.5.725 [doi]
PST - ppublish
SO  - J Leukoc Biol. 1996 May;59(5):725-7. doi: 10.1002/jlb.59.5.725.