PMID- 8656191
OWN - NLM
STAT- MEDLINE
DCOM- 19960730
LR  - 20051117
IS  - 0022-1198 (Print)
IS  - 0022-1198 (Linking)
VI  - 41
IP  - 3
DP  - 1996 May
TI  - PCR-based human leukocyte antigen (HLA) DQ alpha typing of blood stained light 
      and dark blue denim fabric.
PG  - 490-2
AB  - Obtaining typable PCR products from DNA purified from blood stained blue denim 
      has been difficult since inhibitors of PCR in blue denim apparently co-purify 
      with the DNA. Organic and chelex extraction methods were tested for their ability 
      to purify typable DNA from either light or dark blue denim fabric both stained 
      with blood. DNA purified from the light blue denim using either method was 
      successfully used in obtaining correct HLA-DQ alpha typing results. The chelex, 
      but not the organic, procedure was able to yield typable DNA when the dark blue 
      denim was the substrate. Therefore, the chelex method may be more effective than 
      the organic method in preventing compounds that inhibit PCR from co-purifying 
      with the DNA.
FAU - Del Rio, S A
AU  - Del Rio SA
AD  - Armed Forces DNA Identification Laboratory, Armed Forces Institute of Pathology, 
      Rockville, MD, USA.
FAU - Marino, M A
AU  - Marino MA
FAU - Belgrader, P
AU  - Belgrader P
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Forensic Sci
JT  - Journal of forensic sciences
JID - 0375370
RN  - 0 (Coloring Agents)
RN  - 0 (HLA-DQ Antigens)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - *Blood Stains
MH  - *Clothing
MH  - *Coloring Agents
MH  - DNA/*isolation & purification
MH  - Genotype
MH  - HLA-DQ Antigens/*genetics
MH  - Humans
MH  - Polymerase Chain Reaction
EDAT- 1996/05/01 00:00
MHDA- 1996/05/01 00:01
CRDT- 1996/05/01 00:00
PHST- 1996/05/01 00:00 [pubmed]
PHST- 1996/05/01 00:01 [medline]
PHST- 1996/05/01 00:00 [entrez]
PST - ppublish
SO  - J Forensic Sci. 1996 May;41(3):490-2.