PMID- 8656214
OWN - NLM
STAT- MEDLINE
DCOM- 19960801
LR  - 20190116
IS  - 0022-3034 (Print)
IS  - 0022-3034 (Linking)
VI  - 29
IP  - 4
DP  - 1996 Apr
TI  - Brain-derived neurotrophic factor transiently stabilizes silent synapses on 
      developing neuromuscular junctions.
PG  - 503-16
AB  - A general feature of the developing nervous system is the activity-dependent 
      rearrangement of genetically defined, synaptic connections. A parallel process 
      occurs at the developing neuromuscular junction as activity-dependent synapse 
      withdrawal reduces the initial polyneuronal innervation of individual muscle 
      fibers to a mononeuronal innervation within the first few weeks after birth. 
      Because members of the neurotrophin gene family influence motor neuron 
      differentiation and survival, we examined whether or not they also influence 
      synaptic rearrangements in neonatal muscles. We found that treatment with 
      brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), or 
      neurotrophin-4/5 (NT-4/5) causes the transient retention of multiple synaptic 
      contacts on neonatal myofibers. However, the combination of both 
      electrophysiological and histological assays revealed that the majority of such 
      supernumerary synaptic contacts are functionally inactive or "silent." There also 
      occurs an increase in the number of retracting axons. Because BDNF mRNA is 
      expressed in developing muscle and the trkB tyrosine kinase receptor for BDNF is 
      expressed by neonatal motor neurons, our results suggest that BDNF may play an 
      endogenous role in the refinement of synaptic connectivity that occurs in 
      skeletal muscles after birth.
FAU - Kwon, Y W
AU  - Kwon YW
AD  - Department of Cell and Molecular Biology, Northwestern University Medical School, 
      Chicago, Illinois 60611, USA.
FAU - Gurney, M E
AU  - Gurney ME
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Neurobiol
JT  - Journal of neurobiology
JID - 0213640
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neurotrophin 3)
RN  - P658DCA9XD (neurotrophin 4)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Axons/drug effects
MH  - Brain-Derived Neurotrophic Factor
MH  - Electrophysiology
MH  - Female
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Muscle Denervation
MH  - Muscle, Skeletal/innervation/ultrastructure
MH  - Nerve Growth Factors/*pharmacology
MH  - Nerve Tissue Proteins/*pharmacology
MH  - Neuromuscular Junction/drug effects/*embryology/growth & development
MH  - Neurons/cytology/drug effects
MH  - Neurotrophin 3
MH  - Pregnancy
MH  - Synapses/*drug effects/physiology
EDAT- 1996/04/01 00:00
MHDA- 2000/06/20 09:00
CRDT- 1996/04/01 00:00
PHST- 1996/04/01 00:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1996/04/01 00:00 [entrez]
AID - 10.1002/(SICI)1097-4695(199604)29:4<503::AID-NEU7>3.0.CO;2-C [pii]
AID - 10.1002/(SICI)1097-4695(199604)29:4<503::AID-NEU7>3.0.CO;2-C [doi]
PST - ppublish
SO  - J Neurobiol. 1996 Apr;29(4):503-16. doi: 
      10.1002/(SICI)1097-4695(199604)29:4<503::AID-NEU7>3.0.CO;2-C.