PMID- 8656293
OWN - NLM
STAT- MEDLINE
DCOM- 19960730
LR  - 20191101
IS  - 0270-6474 (Print)
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Linking)
VI  - 16
IP  - 12
DP  - 1996 Jun 15
TI  - Expression of NGF and NT3 mRNAs in hippocampal interneurons innervated by the 
      GABAergic septohippocampal pathway.
PG  - 3991-4004
AB  - We used in situ hybridization for the detection of nerve growth factor (NGF), 
      brain-derived neurotrophic factor (BDNF), and neurotrophin 3 (NT3) mRNAs combined 
      with immunocytochemistry against the calcium-binding proteins parvalbumin (PARV), 
      calbindin 28k (CALB), and calretinin (CALR) to determine the expression of 
      neurotrophins in functionally distinct subsets of hippocampal interneurons. Most 
      PARV-immunoreactive neurons in the hippocampus were NGF mRNA-positive (82%), 
      which corresponds to 71% of NGF-positive neurons in the hippocampus proper and in 
      the dentate gyrus (excluding granule cells). In contrast, only a subset of CALB- 
      and CALR-immunoreactive interneurons (24% and 23%, respectively) displayed 
      hybridization signals for NGF. Small subsets of PARV- and CALR-positive cells 
      expressed NT3 mRNA, but we did not find hippocampal interneurons expressing BDNF 
      mRNA. These results show that NGF and NT3 genes are differentially regulated in 
      distinct subsets of GABAergic cells, and these interneurons are a major source of 
      NGF production in the hippocampus. We also addressed whether hippocampal 
      interneurons expressing neurotrophins were targets of the GABAergic 
      septohippocampal pathway. We developed a triple-labeling method that combines 
      anterograde tracing of this pathway by means of Phaseolus vulgaris 
      leucoagglutinin injections, with in situ hybridization for the detection of 
      neurotrophins, and immunocytochemistry for calcium-binding proteins. Virtually 
      every PARV-positive neuron innervated by GABAergic septohippocampal baskets 
      expressed NGF mRNA (86%), whereas 39-59% of CALR- and CALB-positive interneurons 
      that were contacted by GABAergic septohippocampal axons showed NGF gene 
      expression. A small subset of NT3 mRNA-expressing interneurons was also 
      innervated by septohippocampal baskets. These findings show that the GABAergic 
      septohippocampal pathway preferentially terminates on interneurons expressing NGF 
      mRNA, suggesting that this neurotrophic factor might be involved in the 
      specification of this connection and in its maintenance and normal function in 
      the adult brain.
FAU - Rocamora, N
AU  - Rocamora N
AD  - Department of Animal and Plant Cell Biology, Faculty of Biology, University of 
      Barcelona, Spain.
FAU - Pascual, M
AU  - Pascual M
FAU - Acsady, L
AU  - Acsady L
FAU - de Lecea, L
AU  - de Lecea L
FAU - Freund, T F
AU  - Freund TF
FAU - Soriano, E
AU  - Soriano E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Biomarkers)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neurotrophin 3)
RN  - 0 (Phytohemagglutinins)
RN  - 0 (RNA, Messenger)
RN  - 0 (leukoagglutinins, plants)
RN  - 56-12-2 (gamma-Aminobutyric Acid)
SB  - IM
MH  - Afferent Pathways
MH  - Animals
MH  - Axons/chemistry
MH  - Base Sequence
MH  - Biomarkers
MH  - Brain-Derived Neurotrophic Factor
MH  - Cholinergic Fibers/chemistry/physiology
MH  - Hippocampus/*cytology
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Interneurons/cytology/*physiology
MH  - Molecular Sequence Data
MH  - Nerve Growth Factors/analysis/*genetics
MH  - Nerve Tissue Proteins/analysis
MH  - Neurotrophin 3
MH  - Phytohemagglutinins
MH  - RNA, Messenger/physiology
MH  - Rats
MH  - Rats, Wistar
MH  - Septal Nuclei/*cytology
MH  - gamma-Aminobutyric Acid/physiology
PMC - PMC6578621
EDAT- 1996/06/15 00:00
MHDA- 1996/06/15 00:01
PMCR- 1996/12/15
CRDT- 1996/06/15 00:00
PHST- 1996/06/15 00:00 [pubmed]
PHST- 1996/06/15 00:01 [medline]
PHST- 1996/06/15 00:00 [entrez]
PHST- 1996/12/15 00:00 [pmc-release]
AID - 10.1523/JNEUROSCI.16-12-03991.1996 [doi]
PST - ppublish
SO  - J Neurosci. 1996 Jun 15;16(12):3991-4004. doi: 
      10.1523/JNEUROSCI.16-12-03991.1996.