PMID- 8656705
OWN - NLM
STAT- MEDLINE
DCOM- 19960801
LR  - 20131213
IS  - 0025-6196 (Print)
IS  - 0025-6196 (Linking)
VI  - 71
IP  - 7
DP  - 1996 Jul
TI  - Detection of hyperdiploid malignant cells in pleural effusions with 
      chromosome-specific probes and fluorescence in situ hybridization.
PG  - 643-8
AB  - OBJECTIVE: To compare the efficacy of fluorescence in situ hybridization (FISH) 
      by using chromosome-specific probes with standard cytology and cytogenetics for 
      detection of hyperdiploid malignant cells in pleural effusions. MATERIAL AND 
      METHODS: A blind study was done on 26 pleural effusions from 25 patients who had 
      undergone thoracentesis (14 with and 11 without a malignant condition). Cytology, 
      cytogenetics, and FISH with probes specific for chromosomes 7, 8, 12, 18, X, and 
      Y were done on each pleural effusion. For FISH studies, malignant specimens were 
      defined as having 8% or more of cells with hyperdiploidy. RESULTS: Results of 
      cytology and FISH were both normal in each of the 11 patients with benign pleural 
      effusions. Cytogenetic studies were successful in six of these patients: five 
      were chromosomally normal, but one male patient had an abnormal clone that lacked 
      a Y chromosome. Among the 14 patients with malignant pleural effusions, cytology 
      and FISH were abnormal in 8 and 6, respectively. Cytogenetic studies were 
      successful in 11 of these patients, and an abnormal clone was found in 5. 
      CONCLUSION: FISH can detect hyperdiploid malignant cells in pleural effusions and 
      can be useful in the work-up of patients suspected of having malignant pleural 
      effusions.
FAU - Johnson, T M
AU  - Johnson TM
AD  - Mayo Medical School, Rochester, Minnesota 55905, USA.
FAU - Kuffel, D G
AU  - Kuffel DG
FAU - Dewald, G W
AU  - Dewald GW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Mayo Clin Proc
JT  - Mayo Clinic proceedings
JID - 0405543
RN  - 0 (DNA Probes)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - DNA Probes/*genetics
MH  - *Diploidy
MH  - Feasibility Studies
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Pleural Effusion, Malignant/*pathology
EDAT- 1996/07/01 00:00
MHDA- 1996/07/01 00:01
CRDT- 1996/07/01 00:00
PHST- 1996/07/01 00:00 [pubmed]
PHST- 1996/07/01 00:01 [medline]
PHST- 1996/07/01 00:00 [entrez]
AID - S0025-6196(11)63001-2 [pii]
AID - 10.1016/S0025-6196(11)63001-2 [doi]
PST - ppublish
SO  - Mayo Clin Proc. 1996 Jul;71(7):643-8. doi: 10.1016/S0025-6196(11)63001-2.