PMID- 86579
OWN - NLM
STAT- MEDLINE
DCOM- 19790716
LR  - 20170214
IS  - 0022-1554 (Print)
IS  - 0022-1554 (Linking)
VI  - 27
IP  - 1
DP  - 1979 Jan
TI  - Cytofluorometric and cytochemical comparisons of normal and abnormal human cells 
      from the female genital tract.
PG  - 591-5
AB  - Acridine orange staining of exfoliated cells from epithelial tissues facilitates 
      discrimination between normal and abnormal cells: abnormal cells develop highly 
      elevated nuclear fluorescence. Comparisons of acridine orange (AO) staining with 
      propidium iodide (PI) or Feulgen staining have shown that: (a) PI staining also 
      provides highly elevated nuclear fluorescence from abnormal cells; (b) the 
      distributions of nuclear fluorescence following AO or PI staining were usually 
      not significantly different as judged by the Kolmogorov-Smirnov test; (c) 
      fluorescence emission spectra from AO and PI stained cells are consistent with 
      the hypothesis that both fluorochromes bind to DNA within cell nuclei; (d) DNAse 
      treatment of AO stained normal cells eliminates the nuclear fluorescence peak 
      from slit-scan contours; RNAse treatment has no effect on nuclear fluorescence; 
      (e) the distribution of abnormal cell nuclear fluorescence after AO staining is 
      usually, but not always, significantly different from the distribution of 
      abnormal cell nuclear absorbance after Feulgen staining, with relative nuclear 
      fluorescence being greater than relative nuclear absorbance. The hypothesis 
      currently most consistent with these results is that elevated Feulgen DNA content 
      can account for only part of the discrimination provided by AO staining, and that 
      the chromatin within abnormal cells is altered so as to increase accessibility of 
      DNA to intercalating dyes.
FAU - Gill, J E
AU  - Gill JE
FAU - Wheeless, L L Jr
AU  - Wheeless LL Jr
FAU - Hanna-Madden, C
AU  - Hanna-Madden C
FAU - Marisa, R J
AU  - Marisa RJ
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Histochem Cytochem
JT  - The journal of histochemistry and cytochemistry : official journal of the 
      Histochemistry Society
JID - 9815334
RN  - 9007-49-2 (DNA)
RN  - EC 3.1.- (Deoxyribonucleases)
RN  - EC 3.1.- (Ribonucleases)
SB  - IM
MH  - Cell Nucleus/metabolism
MH  - Cervix Uteri/*cytology
MH  - *Cytological Techniques
MH  - DNA/*analysis
MH  - Deoxyribonucleases/metabolism
MH  - Female
MH  - Humans
MH  - Ribonucleases/metabolism
MH  - *Spectrometry, Fluorescence
MH  - *Staining and Labeling
MH  - Uterine Cervical Neoplasms/*analysis
EDAT- 1979/01/01 00:00
MHDA- 2001/03/28 10:01
CRDT- 1979/01/01 00:00
PHST- 1979/01/01 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1979/01/01 00:00 [entrez]
AID - 10.1177/27.1.86579 [doi]
PST - ppublish
SO  - J Histochem Cytochem. 1979 Jan;27(1):591-5. doi: 10.1177/27.1.86579.