PMID- 8658558
OWN - NLM
STAT- MEDLINE
DCOM- 19960730
LR  - 20190819
IS  - 0300-483X (Print)
IS  - 0300-483X (Linking)
VI  - 110
IP  - 1-3
DP  - 1996 Jun 17
TI  - Formyl-methionyl-leucyl-phenylalanine and a calcium ionophore A23187 reverse the 
      inhibition of phorbol myristate acetate-induced oxidative burst by linoleic and 
      oleic acid anilides.
PG  - 39-45
AB  - Linoleic and oleic acid anilides profoundly inhibited the production of reactive 
      oxygen metabolites (ROM) in human polymorphonuclear leukocytes (PMNL) induced by 
      a tumor promoter, phorbol myristate acetate (PMA). The addition of a Ca2+ 
      ionophore, A23187, or a chemotactic peptide, 
      formyl-methionyl-leucyl-phenylalanine (fMLP), readily reversed linoleic and oleic 
      acid anilide-induced inhibiton of PMA-evoked respiratory burst in PMNL without 
      affecting PMA-induced respiratory burst. fMLP or A23187 caused a marked increase 
      in the production of ROM in PMNL that did not produce ROM after their co-exposure 
      to PMA and cis-fatty acid anilides. This suggests a role for Ca2+ in this 
      restoration of respiratory burst activity in PMNL. Oleic and linoleic acid 
      anilides enhanced also respiratory burst in PMNL subsequent to their stimulation 
      with fMLP. Interestingly, corresponding fatty acids, linoleic and oleic acid, 
      also inhibited PMA-induced production of ROM in PMNL, but this inhibition was not 
      reversed by A23187 or fMLP. These findings suggest that the aniline moiety of 
      cis-fatty acids significantly modifies the effects of linoleic and oleic acids in 
      the production of ROM in PMNL. Moreover, free intracellular Ca2+ may play a 
      critical role in the activation of PMNL to produce ROM, and in the modulation of 
      the effects of cis-fatty acid anilides.
FAU - Heiskanen, K M
AU  - Heiskanen KM
AD  - National Public Health Institute, Department of Toxicology, Kuopio, Finland.
FAU - Savolainen, K M
AU  - Savolainen KM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Toxicology
JT  - Toxicology
JID - 0361055
RN  - 0 (Anilides)
RN  - 0 (Carcinogens)
RN  - 0 (Ionophores)
RN  - 0 (Linoleic Acids)
RN  - 0 (Oleic Acids)
RN  - 0 (Reactive Oxygen Species)
RN  - 19878-10-5 (linoleylanilide)
RN  - 37H9VM9WZL (Calcimycin)
RN  - 5429-85-6 (oleoylanilide)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Anilides/*toxicity
MH  - Calcimycin/*pharmacology
MH  - Calcium/metabolism
MH  - Carcinogens/antagonists & inhibitors/toxicity
MH  - Drug Synergism
MH  - Humans
MH  - Ionophores/*pharmacology
MH  - Linoleic Acids/*toxicity
MH  - Luminescent Measurements
MH  - N-Formylmethionine Leucyl-Phenylalanine/*pharmacology
MH  - Neutrophils/cytology/*drug effects/metabolism
MH  - Oleic Acids/*toxicity
MH  - Reactive Oxygen Species/metabolism
MH  - Respiratory Burst/*drug effects
MH  - Tetradecanoylphorbol Acetate/antagonists & inhibitors/toxicity
EDAT- 1996/06/17 00:00
MHDA- 1996/06/17 00:01
CRDT- 1996/06/17 00:00
PHST- 1996/06/17 00:00 [pubmed]
PHST- 1996/06/17 00:01 [medline]
PHST- 1996/06/17 00:00 [entrez]
AID - 0300-483X(96)03320-3 [pii]
AID - 10.1016/0300-483x(96)03320-3 [doi]
PST - ppublish
SO  - Toxicology. 1996 Jun 17;110(1-3):39-45. doi: 10.1016/0300-483x(96)03320-3.