PMID- 8660340 OWN - NLM STAT- MEDLINE DCOM- 19960729 LR - 20131121 IS - 0006-291X (Print) IS - 0006-291X (Linking) VI - 221 IP - 1 DP - 1996 Apr 5 TI - Dehydrogenase binding to the 3'-untranslated region of GLUT1 mRNA. PG - 42-5 AB - Employing RNA gel mobility shift assays we detected specific binding events between several dehydrogenases and various regions of the GLUT1 mRNA 3'-untranslated region. In particular, the enzymes glyceraldehyde 3-phosphate dehydrogenase (G3PDH), lactate dehydrogenase (LDH), and glucose 6-phosphate dehydrogenase (G6PDH) bound to the GLUT1 3'-UTR while isocitrate dehydrogenase (IDH) and glutamate dehydrogenase (GluDH) did not. Comparison of shifts obtained with purified dehydrogenases to those obtained using authentic cell extracts indicate that G3PDH and G6PDH may play a role in the intact cell. FAU - McGowan, K AU - McGowan K AD - Department of Biochemistry, East Carolina University, School of Medicine, Greenville, North Carolina 27834, USA. FAU - Pekala, P H AU - Pekala PH LA - eng GR - GM32892/GM/NIGMS NIH HHS/United States PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, P.H.S. PL - United States TA - Biochem Biophys Res Commun JT - Biochemical and biophysical research communications JID - 0372516 RN - 0 (Glucose Transporter Type 1) RN - 0 (Monosaccharide Transport Proteins) RN - 0 (RNA, Messenger) RN - 0 (Slc2a1 protein, mouse) RN - 9007-49-2 (DNA) RN - EC 1.- (Oxidoreductases) RN - IY9XDZ35W2 (Glucose) SB - IM MH - 3T3 Cells MH - Animals MH - Base Sequence MH - DNA MH - Glucose/metabolism MH - Glucose Transporter Type 1 MH - Mice MH - Molecular Sequence Data MH - Monosaccharide Transport Proteins/*genetics MH - Oxidoreductases/*metabolism MH - RNA, Messenger/*metabolism MH - Substrate Specificity EDAT- 1996/04/05 00:00 MHDA- 1996/04/05 00:01 CRDT- 1996/04/05 00:00 PHST- 1996/04/05 00:00 [pubmed] PHST- 1996/04/05 00:01 [medline] PHST- 1996/04/05 00:00 [entrez] AID - S0006-291X(96)90541-0 [pii] AID - 10.1006/bbrc.1996.0541 [doi] PST - ppublish SO - Biochem Biophys Res Commun. 1996 Apr 5;221(1):42-5. doi: 10.1006/bbrc.1996.0541.