PMID- 8660986
OWN - NLM
STAT- MEDLINE
DCOM- 19970122
LR  - 20161123
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 33
IP  - 2
DP  - 1996 Apr 15
TI  - Visual demonstration of the organization of the human complement C4 and 
      21-hydroxylase genes by high-resolution fluorescence in situ hybridization.
PG  - 321-4
AB  - We analyzed the gene organization in the complement component C4 and 
      21-hydroxylase (21OH) gene region of the human major histocompatibility complex 
      using visual mapping of stretched DNA by multicolor fluorescence in situ 
      hybridization (FISH). Normally, this region contains a duplicated 21OH-C4 gene 
      cluster (21OHB-C4B-21OHA-C4A). Duplication and deletion of one or more copies of 
      the 21OH-C4 gene unit are known to occur frequently. Biotin-labeled cDNA of the 
      C4 gene and digoxigenin-labeled cDNA of the 21OH gene were hybridized to 
      decondensed nuclei of peripheral blood lymphocytes obtained from individuals with 
      various 21OH-C4 haplotypes. Hybridization signals of the C4 and 21OH probes were 
      detected with fluorescein isothiocyanate (green) and rhodamine (red), 
      respectively. Two linear green and red signal clusters were observed in each 
      nucleus showing the normal haplotype. Gene duplication and deletion were 
      visualized as addition and deletion of the signal cluster, respectively. The DNA 
      types of the 21OH-C4 region determined by FISH were concordant with the results 
      previously obtained by conventional molecular studies. Our high-resolution FISH 
      technique is found to be useful for screening gene duplications and deletions.
FAU - Suto, Y
AU  - Suto Y
AD  - Department of Biological Sciences, Graduate School of Science, University of 
      Tokyo, Japan.
FAU - Tokunaga, K
AU  - Tokunaga K
FAU - Watanabe, Y
AU  - Watanabe Y
FAU - Hirai, M
AU  - Hirai M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (Complement C4)
RN  - EC 1.14.14.16 (Steroid 21-Hydroxylase)
SB  - IM
MH  - Complement C4/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Steroid 21-Hydroxylase/*genetics
EDAT- 1996/04/15 00:00
MHDA- 1996/04/15 00:01
CRDT- 1996/04/15 00:00
PHST- 1996/04/15 00:00 [pubmed]
PHST- 1996/04/15 00:01 [medline]
PHST- 1996/04/15 00:00 [entrez]
AID - S0888-7543(96)90202-5 [pii]
AID - 10.1006/geno.1996.0202 [doi]
PST - ppublish
SO  - Genomics. 1996 Apr 15;33(2):321-4. doi: 10.1006/geno.1996.0202.