PMID- 8661115
OWN - NLM
STAT- MEDLINE
DCOM- 19960930
LR  - 20070717
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 35
IP  - 1
DP  - 1996 Jul 1
TI  - Characterization of msim, a murine homologue of the Drosophila sim transcription 
      factor.
PG  - 144-55
AB  - Mutations in the Drosophila single-minded (sim) gene result in loss of precursor 
      cells that give rise to midline cells of the embryonic central nervous system. 
      During the course of an exon-trapping strategy aimed at identifying transcripts 
      that contribute to the etiology and pathophysiology of Down syndrome, we 
      identified a human exon from the Down syndrome critical region showing 
      significant homology to the Drosophila sim gene. Using a cross-hybridization 
      approach, we have isolated a murine homolog of the Drosophila sim gene, which we 
      designated msim. Nucleotide and predicted amino acid sequence analyses of msim 
      cDNA clones indicate that this gene encodes a member of the 
      basic-helix-loop-helix class of transcription factors. The murine and Drosophila 
      proteins share 88% residues within the basic-helix-loop-helix domain, with an 
      overall homology of 92%. In addition, the N-terminal domain of MSIM contains two 
      PAS dimerization motifs also featured in the Drosophila sim gene product, as well 
      as a small number of other transcription factors. Northern blot analysis of adult 
      murine tissues revealed that the msim gene produces a single mRNA species of 
      approximately 4 kb expressed in a small number of tissues, with the highest 
      levels in the kidneys and lower levels present in skeletal muscle, lung, testis, 
      brain, and heart. In situ hybridization experiments demonstrate that msim is also 
      expressed in early fetal development in the central nervous system and in 
      cartilage primordia. The characteristics of the msim gene are consistent with its 
      putative function as a transcriptional regulator.
FAU - Moffett, P
AU  - Moffett P
AD  - Department of Biochemistry, McGill University, 3655 Drummond Street, Montreal, 
      Quebec, H3G 1Y6, Canada.
FAU - Dayo, M
AU  - Dayo M
FAU - Reece, M
AU  - Reece M
FAU - McCormick, M K
AU  - McCormick MK
FAU - Pelletier, J
AU  - Pelletier J
LA  - eng
SI  - GENBANK/U42554
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (Basic Helix-Loop-Helix Transcription Factors)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Fetal Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (SIM2 protein, human)
RN  - 0 (Sim2 protein, mouse)
RN  - 0 (Transcription Factors)
RN  - 0 (sim protein, Drosophila)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Basic Helix-Loop-Helix Transcription Factors
MH  - Chromosome Walking
MH  - DNA-Binding Proteins/biosynthesis/*genetics
MH  - Disease Models, Animal
MH  - Down Syndrome/genetics
MH  - Drosophila Proteins
MH  - Drosophila melanogaster/*genetics
MH  - Embryonic and Fetal Development
MH  - Fetal Proteins/biosynthesis/genetics
MH  - Gene Expression Regulation, Developmental
MH  - *Genes
MH  - Genes, Insect
MH  - Helix-Loop-Helix Motifs/genetics
MH  - Humans
MH  - Mice/*genetics
MH  - Mice, Mutant Strains
MH  - Molecular Sequence Data
MH  - Nerve Tissue Proteins/biosynthesis/genetics
MH  - Nuclear Proteins/biosynthesis/*genetics
MH  - Organ Specificity
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Species Specificity
MH  - Transcription Factors/biosynthesis/*genetics
MH  - Transcription, Genetic
MH  - Trisomy
EDAT- 1996/07/01 00:00
MHDA- 1996/07/01 00:01
CRDT- 1996/07/01 00:00
PHST- 1996/07/01 00:00 [pubmed]
PHST- 1996/07/01 00:01 [medline]
PHST- 1996/07/01 00:00 [entrez]
AID - S0888-7543(96)90333-X [pii]
AID - 10.1006/geno.1996.0333 [doi]
PST - ppublish
SO  - Genomics. 1996 Jul 1;35(1):144-55. doi: 10.1006/geno.1996.0333.