PMID- 8662247
OWN - NLM
STAT- MEDLINE
DCOM- 19960904
LR  - 20221207
IS  - 0009-5915 (Print)
IS  - 0009-5915 (Linking)
VI  - 104
IP  - 8
DP  - 1996 Jun
TI  - High resolution physical mapping of 45S (5.8S, 18S and 25S) rDNA gene loci in the 
      tomato genome using a combination of karyotyping and FISH of pachytene 
      chromosomes.
PG  - 545-50
AB  - Karyotyping in combination with fluorescence in situ hybridization (FISH) on 
      tomato pachytene chromosomes allowed identification and mapping of a major 45S 
      (5.8S, 18S and 25S) rDNA site on the satellite of 2S and four minor loci, each at 
      a proximal knob on 2L, 6S, 9S and 11S. Thus, the 45S rDNA loci are all located in 
      heterochromatic regions. The five 45S sites are all transcriptionally active as 
      evidenced by a maximum of ten nucleoli in meiotic cells at telophase or 
      interphase. The 45S rDNA loci, as well as the 5S rDNA locus on 1S, were 
      highlighted by chromomycin A3, a GC-specific DNA ligand; this result is 
      consistent with the high GC content of the rDNA genes. Satellite size varied 
      dramatically between genotypes. Enzymatic maceration of tomato anthers followed 
      by squashing in acetocarmine produced high quality chromosomal preparations and 
      subsequent FISH images by reducing the strong autofluorescence inherent in the 
      nucleolus and cytoplasm of tomato meiotic cells. Our protocol has potential in 
      the construction of an integrated cytological, classical and molecular map of 
      tomato.
FAU - Xu, J
AU  - Xu J
AD  - Department of Plant Breeding and Biometry, Cornell University, Ithaca, NY 
      14853-1902, USA.
FAU - Earle, E D
AU  - Earle ED
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Austria
TA  - Chromosoma
JT  - Chromosoma
JID - 2985138R
RN  - 0 (Chromomycins)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Heterochromatin)
RN  - 0 (Indoles)
RN  - 0 (aceto-carmine)
RN  - 47165-04-8 (DAPI)
RN  - CID8Z8N95N (Carmine)
SB  - IM
MH  - Carmine/analogs & derivatives/chemistry
MH  - Cell Nucleolus/genetics
MH  - Chromomycins/chemistry
MH  - Chromosome Mapping/*methods
MH  - Chromosomes
MH  - DNA, Ribosomal/*genetics
MH  - *Genome, Plant
MH  - Heterochromatin
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Indoles/chemistry
MH  - Karyotyping/*methods
MH  - Solanum lycopersicum/*genetics
MH  - Silver Staining
MH  - Staining and Labeling
MH  - Transcription, Genetic
EDAT- 1996/06/01 00:00
MHDA- 1996/06/01 00:01
CRDT- 1996/06/01 00:00
PHST- 1996/06/01 00:00 [pubmed]
PHST- 1996/06/01 00:01 [medline]
PHST- 1996/06/01 00:00 [entrez]
AID - 10.1007/BF00352294 [doi]
PST - ppublish
SO  - Chromosoma. 1996 Jun;104(8):545-50. doi: 10.1007/BF00352294.