PMID- 8662803
OWN - NLM
STAT- MEDLINE
DCOM- 19960815
LR  - 20211203
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 271
IP  - 22
DP  - 1996 May 31
TI  - Aggregation of the FcepsilonRI on mast cells stimulates c-Jun amino-terminal 
      kinase activity. A response inhibited by wortmannin.
PG  - 12762-6
AB  - Aggregation of the high-affinity Fc receptors for immunoglobulin E (IgE) 
      (FcepsilonRI) on the surface of mast cells initiates intracellular signal 
      transduction pathways including the tyrosine phosphorylation of cellular 
      proteins, phosphoinositide hydrolysis, an increase in intracellular calcium, and 
      protein kinase C activation. These signals are believed to be involved in the 
      exocytic release of inflammatory mediators such as vasoactive amines, cytokines, 
      and lipid metabolites. However, the downstream consequences of these early 
      activation events are not well defined. One exception is the activation of the 
      extracellular signal-regulated kinases/mitogen-activated protein kinases. One 
      member of the mitogen-activated protein kinase superfamily, designated c-Jun 
      amino-terminal kinase (JNK), has been recently identified. JNK is activated 
      following dual phosphorylation at a Thr-Pro-Tyr motif in response to diverse 
      stimuli including tumor necrosis factor-alpha, heat shock, or ultraviolet 
      irradiation. We found that JNK was strongly activated by antigen cross-linking in 
      a mouse mast cell line passively sensitized with ovalbumin-specific IgE. 
      Anti-mouse IgE antibody also activated JNK. MEK kinase 1 (MEKK1) which activates 
      the JNK activator, JNK kinase (JNKK), was similarly activated by antigen 
      stimulation. JNK but not p42(erk2) activation induced by antigen was 
      significantly inhibited in the presence of wortmannin, a known inhibitor of 
      phosphatidylinositol 3-kinase. These results indicate that in response to the 
      aggregation of FcepsilonRI on mast cells, phosphatidylinositol 3-kinase 
      activation is involved in the stimulation of the MEKK1, JNKK, JNK pathway.
FAU - Ishizuka, T
AU  - Ishizuka T
AD  - Division of Basic Sciences, Department of Pediatrics, and the Program in 
      Molecular Signal Transduction, National Jewish Center for Immunology and 
      Respiratory Medicine, Denver, Colorado 80206, USA.
FAU - Oshiba, A
AU  - Oshiba A
FAU - Sakata, N
AU  - Sakata N
FAU - Terada, N
AU  - Terada N
FAU - Johnson, G L
AU  - Johnson GL
FAU - Gelfand, E W
AU  - Gelfand EW
LA  - eng
GR  - AI HL-36577/AI/NIAID NIH HHS/United States
GR  - DK-37871/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Androstadienes)
RN  - 0 (Antigens)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Protein Kinase Inhibitors)
RN  - 0 (Receptors, IgE)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
RN  - EC 2.7.11.25 (MAP Kinase Kinase Kinase 1)
RN  - EC 2.7.11.25 (Map3k1 protein, mouse)
RN  - EC 2.7.12.2 (MAP Kinase Kinase 4)
RN  - EC 2.7.12.2 (Mitogen-Activated Protein Kinase Kinases)
RN  - XVA4O219QW (Wortmannin)
SB  - IM
MH  - Amino Acid Sequence
MH  - Androstadienes/*pharmacology
MH  - Animals
MH  - Antigens/pharmacology
MH  - Calcium-Calmodulin-Dependent Protein Kinases/metabolism
MH  - Enzyme Activation
MH  - Enzyme Inhibitors/*pharmacology
MH  - Immunoglobulin E/immunology
MH  - *JNK Mitogen-Activated Protein Kinases
MH  - MAP Kinase Kinase 4
MH  - *MAP Kinase Kinase Kinase 1
MH  - Mast Cells/*metabolism
MH  - Mice
MH  - Mitogen-Activated Protein Kinase 1
MH  - *Mitogen-Activated Protein Kinase Kinases
MH  - Molecular Sequence Data
MH  - Phosphorylation
MH  - Protein Kinase Inhibitors
MH  - Protein Kinases/*metabolism
MH  - Protein Serine-Threonine Kinases/metabolism
MH  - Receptors, IgE/*metabolism
MH  - Wortmannin
EDAT- 1996/05/31 00:00
MHDA- 1996/05/31 00:01
CRDT- 1996/05/31 00:00
PHST- 1996/05/31 00:00 [pubmed]
PHST- 1996/05/31 00:01 [medline]
PHST- 1996/05/31 00:00 [entrez]
AID - S0021-9258(18)41426-3 [pii]
AID - 10.1074/jbc.271.22.12762 [doi]
PST - ppublish
SO  - J Biol Chem. 1996 May 31;271(22):12762-6. doi: 10.1074/jbc.271.22.12762.