PMID- 8663314
OWN - NLM
STAT- MEDLINE
DCOM- 19960912
LR  - 20210210
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 271
IP  - 29
DP  - 1996 Jul 19
TI  - Molecular cloning and functional characterization of a novel human CC chemokine 
      receptor (CCR5) for RANTES, MIP-1beta, and MIP-1alpha.
PG  - 17161-6
AB  - Chemokines affect leukocyte chemotactic and activation activities through 
      specific G protein-coupled receptors. In an effort to map the closely linked CC 
      chemokine receptor genes, we identified a novel chemokine receptor encoded 18 
      kilobase pairs downstream of the monocyte chemoattractant protein-1 (MCP-1) 
      receptor (CCR2) gene on human chromosome 3p21. The deduced amino acid sequence of 
      this novel receptor, designated CCR5, is most similar to CCR2B, sharing 71% 
      identical residues. Transfected cells expressing the receptor bind RANTES 
      (regulated on activation normal T cell expressed), MIP-1beta, and MIP-1alpha with 
      high affinity and generate inositol phosphates in response to these chemokines. 
      This same combination of chemokines has recently been shown to potently inhibit 
      human immunodeficiency virus replication in human peripheral blood leukocytes 
      (Cocchi, F., DeVico, A. L., Garzino-Demo, A., Arya, S. K., Gallo, R. C., and 
      Lusso, P.(1995) Science 270, 1811-1815). CCR5 is expressed in lymphoid organs 
      such as thymus and spleen, as well as in peripheral blood leukocytes, including 
      macrophages and T cells, and is the first example of a human chemokine receptor 
      that signals in response to MIP-1beta.
FAU - Raport, C J
AU  - Raport CJ
AD  - ICOS Corporation, Bothell, Washington 98021, USA.
FAU - Gosling, J
AU  - Gosling J
FAU - Schweickart, V L
AU  - Schweickart VL
FAU - Gray, P W
AU  - Gray PW
FAU - Charo, I F
AU  - Charo IF
LA  - eng
SI  - GENBANK/U54994
GR  - HL52773/HL/NHLBI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Chemokine CCL3)
RN  - 0 (Chemokine CCL4)
RN  - 0 (Chemokine CCL5)
RN  - 0 (DNA Primers)
RN  - 0 (Growth Inhibitors)
RN  - 0 (Macrophage Inflammatory Proteins)
RN  - 0 (Monokines)
RN  - 0 (Receptors, CCR5)
RN  - 0 (Receptors, Cytokine)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Chemokine CCL3
MH  - Chemokine CCL4
MH  - Chemokine CCL5/*metabolism
MH  - Chromosome Mapping
MH  - Chromosomes, Artificial, Yeast
MH  - *Chromosomes, Human, Pair 3
MH  - Cloning, Molecular
MH  - DNA Primers
MH  - Female
MH  - Gene Library
MH  - Growth Inhibitors/metabolism
MH  - Humans
MH  - Kinetics
MH  - Macrophage Inflammatory Proteins
MH  - Male
MH  - Molecular Sequence Data
MH  - Monokines/*metabolism
MH  - Multigene Family
MH  - Organ Specificity
MH  - Polymerase Chain Reaction
MH  - Receptors, CCR5
MH  - Receptors, Cytokine/chemistry/genetics/*metabolism
MH  - Restriction Mapping
MH  - Sequence Homology, Amino Acid
EDAT- 1996/07/19 00:00
MHDA- 1996/07/19 00:01
CRDT- 1996/07/19 00:00
PHST- 1996/07/19 00:00 [pubmed]
PHST- 1996/07/19 00:01 [medline]
PHST- 1996/07/19 00:00 [entrez]
AID - S0021-9258(18)31312-7 [pii]
AID - 10.1074/jbc.271.29.17161 [doi]
PST - ppublish
SO  - J Biol Chem. 1996 Jul 19;271(29):17161-6. doi: 10.1074/jbc.271.29.17161.