PMID- 8664575
OWN - NLM
STAT- MEDLINE
DCOM- 19960802
LR  - 20190729
IS  - 1060-3271 (Print)
IS  - 1060-3271 (Linking)
VI  - 78
IP  - 6
DP  - 1995 Nov-Dec
TI  - Optimization of a liquid chromatographic method for determination of malachite 
      green and its metabolites in fish tissues.
PG  - 1388-94
AB  - A liquid chromatographic (LC) method was adapted and optimized for the 
      determination of malachite green and its metabolites in fish plasma and muscle. 
      Residues in plasma were extracted with acetonitrile, the extract was evaporated 
      to dryness, and residues were resolubilized for LC analysis. Residues in muscle 
      were extracted with an acetonitrile-acetate buffer mixture, reextracted with 
      acetonitrile, and partitioned into methylene chloride with final cleanup on 
      alumni and propylsulfonic acid solid-phase extraction columns. Residue levels 
      were determined by using an LC cyano column with a PbO2 postcolumn and visible 
      detection (618 nm). Overall mean recoveries of parent malachite green (MG-C) and 
      its major metabolite, leuco-malachite green (MG-L), from plasma were 93 and 
      87%,respectively, at fortification levels ranging from 25 to 250 ppb. Overall 
      mean recoveries of MG-C and MG-L from muscle were 85 and 95%, respectively, at 
      fortification levels ranging from 5 to 100 ppb. Relative standard deviations 
      (RSDs) of recoveries at all fortification levels ranged from 3.9 to 7.0% for 
      plasma and from 2.1 to 5.2% for muscle. The method was applied to incurred 
      residues in tissues sampled from catfish after waterborne exposure to [14C]MG-C. 
      Mean recoveries of total radioactive residues in plasma and muscle throughout the 
      extraction and cleanup process were 88 and 87%, respectively, and corresponding 
      RSDs for MG-C and MG-L were in the same range as those for fortified tissues. 
      MG-L was confirmed as the major metabolite of MG-C in catfish.
FAU - Plakas, S M
AU  - Plakas SM
AD  - U.S. Food and Drug Administration, Gulf Coast Seafood Laboratory, Dauphin Island, 
      AL 36528, USA.
FAU - el Said, K R
AU  - el Said KR
FAU - Stehly, G R
AU  - Stehly GR
FAU - Roybal, J E
AU  - Roybal JE
LA  - eng
PT  - Journal Article
PL  - England
TA  - J AOAC Int
JT  - Journal of AOAC International
JID - 9215446
RN  - 0 (Aniline Compounds)
RN  - 0 (Fungicides, Industrial)
RN  - 0 (Pesticide Residues)
RN  - 0 (Rosaniline Dyes)
RN  - 12058M7ORO (malachite green)
RN  - 8U61G37Z20 (leucomalachite green)
SB  - IM
MH  - Aniline Compounds/*analysis
MH  - Animals
MH  - Chromatography, Liquid/*methods
MH  - Fungicides, Industrial/*analysis/metabolism
MH  - Ictaluridae/blood/*metabolism
MH  - Muscles/*metabolism
MH  - Pesticide Residues
MH  - Rosaniline Dyes/*analysis/metabolism
EDAT- 1995/11/01 00:00
MHDA- 1995/11/01 00:01
CRDT- 1995/11/01 00:00
PHST- 1995/11/01 00:00 [pubmed]
PHST- 1995/11/01 00:01 [medline]
PHST- 1995/11/01 00:00 [entrez]
PST - ppublish
SO  - J AOAC Int. 1995 Nov-Dec;78(6):1388-94.