PMID- 8666326
OWN - NLM
STAT- MEDLINE
DCOM- 19960802
LR  - 20061115
IS  - 0270-9139 (Print)
IS  - 0270-9139 (Linking)
VI  - 23
IP  - 4
DP  - 1996 Apr
TI  - Comparison of glyceraldehyde-3-phosphate dehydrogenase and 28s-ribosomal RNA gene 
      expression in human hepatocellular carcinoma.
PG  - 734-7
AB  - The gene responsible for transcribing glyceraldehyde-3-phosphate dehydrogenase 
      (GAPDH) is commonly used as a reporter gene to estimate the amount of RNA present 
      in Northern analyses. However, recent data suggest that GAPDH gene expression may 
      vary with the extent of cell proliferation and differentiation. 28S-ribosomal RNA 
      (28S-rRNA) has also been employed to normalize Northern blots prepared with total 
      RNA. In the present study, we compared the expression of GAPDH messenger RNA 
      (mRNA) with 28S-rRNA by Northern blot analyses in human hepatocellular carcinoma 
      tissues (HCC) and adjacent non-HCC tissues from eight patients with chronic viral 
      hepatitis-induced cirrhosis and normal liver tissue from eight healthy control 
      subjects. The results of the study revealed that GAPDH mRNA levels in HCC were 
      significantly higher (14X-16x) than those in adjacent non-HCC and normal liver 
      tissues. Conversely, 28S-rRNA levels did not vary among HCC, adjacent non-HCC, 
      and normal liver tissues. We also demonstrated that the 28S-RNA signal was 
      proportional to the amount of RNA loaded. These findings indicate that 28S-rRNA, 
      rather than GAPDH mRNA, should be used as RNA loading controls for Northern blot 
      analyses involving HCC and nontumor tissues. The findings also raise the 
      possibility that GAPDH mRNA gene expression might serve as a diagnostic indicator 
      for human HCC.
FAU - Gong, Y
AU  - Gong Y
AD  - Liver Diseases Unit, Department of Medicine, University of Manitoba, Winnipeg, 
      Canada.
FAU - Cui, L
AU  - Cui L
FAU - Minuk, G Y
AU  - Minuk GY
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Hepatology
JT  - Hepatology (Baltimore, Md.)
JID - 8302946
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Ribosomal, 28S)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
SB  - IM
CIN - Hepatology. 1997 Sep;26(3):803. PMID: 9303519
MH  - Adult
MH  - Carcinoma, Hepatocellular/*metabolism
MH  - Female
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/*genetics
MH  - Humans
MH  - Liver Neoplasms/*metabolism
MH  - Male
MH  - Middle Aged
MH  - RNA, Messenger/analysis
MH  - RNA, Ribosomal, 28S/*genetics
EDAT- 1996/04/01 00:00
MHDA- 1996/04/01 00:01
CRDT- 1996/04/01 00:00
PHST- 1996/04/01 00:00 [pubmed]
PHST- 1996/04/01 00:01 [medline]
PHST- 1996/04/01 00:00 [entrez]
AID - S027091399600119X [pii]
AID - 10.1002/hep.510230413 [doi]
PST - ppublish
SO  - Hepatology. 1996 Apr;23(4):734-7. doi: 10.1002/hep.510230413.