PMID- 8666390
OWN - NLM
STAT- MEDLINE
DCOM- 19960807
LR  - 20061115
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 29
IP  - 2
DP  - 1995 Sep 20
TI  - Construction and characterization of a bovine bacterial artificial chromosome 
      library.
PG  - 413-25
AB  - A bacterial artificial chromosome (BAC) library has been constructed for use in 
      bovine genome mapping using constructed for use in bovine genome mapping using 
      the pBeloBAC11 vector. Currently, the library consists of 23,040 clones, which 
      achieves a 70% probability (P=0.70) of the library containing a specific unique 
      DNA sequence. Sixty thousand clones, or about three haploid bovine genomes, will 
      be required to achieve a 95% probability (P=0.95) of containing a unique 
      sequence. An average insert size of 146 kb was estimated from the analysis of 77 
      randomly selected BAC clones produced by one or two rounds of size selection. The 
      bovine DNA inserts proved to be very stable for at least 100 cell generations. No 
      chimeric clones were detected among 11 large, size-selected BAC clones using 
      fluorescence in situ hybridization (FISH) on metaphase bovine chromosomes. 
      Thirty-three of 46 (72%) sequences were present in the library in at least one 
      copy, which is consistent with the estimated 70% probability of this library 
      containing a unique DNA sequence. A BAC clone as sequence-tagged sites for 
      genetic mapping. These markers cosegregated, and no recombinants were detected in 
      193 informative meioses. Plasmid end rescue and the inverse polymerase chain 
      reaction methods were used to rescue both ends of this BAC clone, and chromosome 
      walking was performed using PCR primers designed within the end region sequences. 
      Based on our experimental results, the BAC system provides a very useful tool for 
      complex genome analysis.
FAU - Cai, L
AU  - Cai L
AD  - Department of Animal Science, Texas A&M University, College Station 77843, USA.
FAU - Taylor, J F
AU  - Taylor JF
FAU - Wing, R A
AU  - Wing RA
FAU - Gallagher, D S
AU  - Gallagher DS
FAU - Woo, S S
AU  - Woo SS
FAU - Davis, S K
AU  - Davis SK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (DNA, Satellite)
RN  - 0 (Genetic Markers)
RN  - 9007-49-2 (DNA)
RN  - EC 1.1.- (3-Hydroxysteroid Dehydrogenases)
RN  - EC 1.1.1.- (delta(5)-3 beta-hydroxysteroid dehydrogenase)
SB  - IM
MH  - 3-Hydroxysteroid Dehydrogenases/genetics
MH  - Animals
MH  - Base Sequence
MH  - Cattle/*genetics
MH  - Chimera
MH  - *Chromosome Mapping
MH  - Chromosome Walking
MH  - Chromosomes, Bacterial
MH  - Cloning, Molecular/methods
MH  - DNA/blood/genetics/isolation & purification
MH  - DNA, Satellite/genetics
MH  - Electrophoresis, Agar Gel
MH  - *Gene Library
MH  - Genetic Markers
MH  - Genetic Vectors
MH  - Genome
MH  - Haploidy
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction/methods
MH  - Restriction Mapping
MH  - X Chromosome
EDAT- 1995/09/20 00:00
MHDA- 1995/09/20 00:01
CRDT- 1995/09/20 00:00
PHST- 1995/09/20 00:00 [pubmed]
PHST- 1995/09/20 00:01 [medline]
PHST- 1995/09/20 00:00 [entrez]
AID - S0888-7543(85)79986-7 [pii]
AID - 10.1006/geno.1995.9986 [doi]
PST - ppublish
SO  - Genomics. 1995 Sep 20;29(2):413-25. doi: 10.1006/geno.1995.9986.