PMID- 8667428
OWN - NLM
STAT- MEDLINE
DCOM- 19960807
LR  - 20190512
IS  - 0027-8874 (Print)
IS  - 0027-8874 (Linking)
VI  - 88
IP  - 14
DP  - 1996 Jul 17
TI  - B-cell non-Hodgkin's lymphoma: evidence for the t (14;18) translocation in all 
      hematopoietic cell lineages.
PG  - 973-9
AB  - BACKGROUND: B cells of patients with non-Hodgkin's lymphoma (B-NHL) harbor 
      specific chromosomal translocations, including t(14;18), the most common 
      aberration found in this disease. The translocation involves the immunoglobulin 
      (Ig) heavy-chain joining (JH) region gene on chromosome 14 and the BCL2 gene on 
      chromosome 18, resulting in dysregulated expression of the BCL2 gene. The 
      t(14;18) translocation has been thought to occur in the pre-B-cell stage, during 
      the first event of Ig gene rearrangement. PURPOSE: This study was conducted to 
      investigate the potential involvement of nonlymphoid lineages in B-NHL. METHODS: 
      We studied the t(14;18) translocation and other frequently occurring 
      translocations in total bone marrow aspirates of 10 patients with B-NHL, with the 
      use of the fluorescence in situ hybridization (FISH) technique. We also performed 
      cytogenetic analyses on representative bone marrow aspirates from the patients. 
      Moreover, to define which of the major cell lineages present in the bone marrow 
      carry the t(14;18) translocation, we used a series of monoclonal antibodies 
      together with fluorescence-activated cell sorter (FACS) analyses to purify cells 
      positive for CD3 (T cells), CD19 (B cells), CD10 (CALLA-positive cells), CD41a 
      (megakaryocytic cells), CD13 (myeloid cells), and glycophorin A (erythroid 
      cells). The cells of each subgroup underwent FISH analysis with the use of JH and 
      BCL2 probes to detect the t(14;18) translocation. Bone marrow samples obtained 
      from five healthy donors served as controls. RESULTS: Bone marrow cells from 
      eight of the 10 patients studied carried the t(14;18) translocation. When 
      present, the translocation was observed in many or even all of the cell lineages 
      (lymphoid, myeloid, megakaryocytic, and erythroid) present in the bone marrow, 
      including peripheral blood progenitor stem cells; for seven of the eight patients 
      carrying the translocation, it was found in 96%-100% of the unfractionated bone 
      marrow cells as well as in all of the FACS-purified cell fractions in which it 
      could be detected or studied. Conventional cytogenetic analyses performed on 
      representative bone marrow aspirates confirmed the results obtained by FISH 
      analysis. Cells in control bone marrow samples obtained from the five healthy 
      donors were negative for the t(14;18) translocation by FISH analysis. 
      CONCLUSIONS: Our findings indicate that the t(14;18) translocation most probably 
      occurs in a very early multilineage progenitor stem cell. IMPLICATIONS: Given 
      that the t(14;18) chromosomal translocation was found in all types of bone marrow 
      cells when only the B cells were malignant, our results suggest that this 
      translocation is not sufficient to induce neoplastic transformation. This finding 
      underscores the need for the development of new approaches for the detection and 
      surveillance of B-NHL.
FAU - Yarkoni, S
AU  - Yarkoni S
AD  - Department of Cellular Biochemistry, Hebrew University-Hadassah Medical School, 
      Jerusalem, Israel.
FAU - Lishner, M
AU  - Lishner M
FAU - Tangi, I
AU  - Tangi I
FAU - Nagler, A
AU  - Nagler A
FAU - Lorberboum-Galski, H
AU  - Lorberboum-Galski H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Natl Cancer Inst
JT  - Journal of the National Cancer Institute
JID - 7503089
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Neoplasm)
SB  - IM
CIN - J Natl Cancer Inst. 1997 Jan 1;89(1):93-5. PMID: 8978417
MH  - *Chromosomes, Human, Pair 14
MH  - *Chromosomes, Human, Pair 18
MH  - DNA Probes
MH  - DNA, Neoplasm/genetics
MH  - *Hematopoietic Stem Cells
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphoma, B-Cell/*genetics
MH  - *Translocation, Genetic
EDAT- 1996/07/17 00:00
MHDA- 1996/07/17 00:01
CRDT- 1996/07/17 00:00
PHST- 1996/07/17 00:00 [pubmed]
PHST- 1996/07/17 00:01 [medline]
PHST- 1996/07/17 00:00 [entrez]
AID - 10.1093/jnci/88.14.973 [doi]
PST - ppublish
SO  - J Natl Cancer Inst. 1996 Jul 17;88(14):973-9. doi: 10.1093/jnci/88.14.973.