PMID- 8668145
OWN - NLM
STAT- MEDLINE
DCOM- 19960808
LR  - 20210526
IS  - 0270-7306 (Print)
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Linking)
VI  - 16
IP  - 7
DP  - 1996 Jul
TI  - Interleukin-11 mRNA stabilization in phorbol ester-stimulated primate bone marrow 
      stromal cells.
PG  - 3300-7
AB  - 12-O-Tetradecanoylphorbol-13-acetate (TPA) stimulation of PU-34 cells, a primate 
      bone marrow stromal cell line, resulted in a prolonged elevation of 
      interleukin-11 (IL-11) mRNA, which can be inhibited by protein synthesis 
      inhibitors. Nuclear run-on assays and actinomycin D experiments demonstrated that 
      the up-regulation of IL-11 gene expression is mainly controlled at the 
      posttranscriptional level through the protein kinase C (PKC) pathway. Inhibition 
      of PKC activity by calphostin C generated an IL-11 mRNA degradation intermediate 
      in TPA-stimulated PU-34 cells. This intermediate retains the 5' untranslated 
      region (5'UTR) and coding region of the IL-11 mRNA but has lost the poly(A) tail 
      and the 3'UTR. The mechanisms underlying IL-11 mRNA stabilization were further 
      investigated by transfections with a variety of chimeric IL-11 constructs and 
      deletion mutants. Two important observations were made from these transient 
      expression experiments: (i) the same 3'UTR of IL-11 mRNA shown to confer 
      instability in one chimeric transcript may not function as a destabilizer in 
      another chimeric RNA, and (ii) the 5'UTR, coding region, and 3'UTR all contribute 
      to IL-11 mRNA decay, and labile IL-11 deletion transcripts are not necessarily 
      stabilized by TPA stimulation. Our study suggests that multiple regions within 
      the IL-11 mRNA are involved in TPA-stimulated IL-11 mRNA stabilization, possibly 
      through a unique RNA folding conformation involving interactions of various RNA 
      sequences within the IL-11 mRNA molecule.
FAU - Yang, L
AU  - Yang L
AD  - Department of Biochemistry and Molecular Biology, Walter Oncology Center, Indiana 
      University School of Medicine, Indianapolis, 46202, USA.
FAU - Steussy, C N
AU  - Steussy CN
FAU - Fuhrer, D K
AU  - Fuhrer DK
FAU - Hamilton, J
AU  - Hamilton J
FAU - Yang, Y C
AU  - Yang YC
LA  - eng
GR  - R01DK43105/DK/NIDDK NIH HHS/United States
GR  - R01HL48819/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (DNA Probes)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Interleukin-11)
RN  - 0 (Isoflavones)
RN  - 0 (Isoquinolines)
RN  - 0 (Naphthalenes)
RN  - 0 (Piperazines)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 1CC1JFE158 (Dactinomycin)
RN  - 84477-87-2 (1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine)
RN  - DH2M523P0H (Genistein)
RN  - EC 1.13.12.- (Luciferases)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - I271P23G24 (calphostin C)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
MH  - Animals
MH  - *Bone Marrow Cells
MH  - Cell Line
MH  - Cell Nucleus/metabolism
MH  - DNA Probes
MH  - Dactinomycin/pharmacology
MH  - Enzyme Inhibitors/pharmacology
MH  - *Gene Expression Regulation/drug effects
MH  - Genistein
MH  - Interleukin-11/*biosynthesis
MH  - Isoflavones/pharmacology
MH  - Isoquinolines/pharmacology
MH  - Kinetics
MH  - Luciferases/biosynthesis
MH  - Naphthalenes/pharmacology
MH  - Open Reading Frames
MH  - Piperazines/pharmacology
MH  - Primates
MH  - Protein Kinase C/antagonists & inhibitors
MH  - *RNA Processing, Post-Transcriptional
MH  - RNA, Messenger/*metabolism
MH  - Recombinant Fusion Proteins/biosynthesis
MH  - Stromal Cells/immunology
MH  - Tetradecanoylphorbol Acetate/*pharmacology
MH  - Time Factors
MH  - Transcription, Genetic
MH  - Transfection
PMC - PMC231324
EDAT- 1996/07/01 00:00
MHDA- 1996/07/01 00:01
PMCR- 1996/07/01
CRDT- 1996/07/01 00:00
PHST- 1996/07/01 00:00 [pubmed]
PHST- 1996/07/01 00:01 [medline]
PHST- 1996/07/01 00:00 [entrez]
PHST- 1996/07/01 00:00 [pmc-release]
AID - 10.1128/MCB.16.7.3300 [doi]
PST - ppublish
SO  - Mol Cell Biol. 1996 Jul;16(7):3300-7. doi: 10.1128/MCB.16.7.3300.