PMID- 8670281
OWN - NLM
STAT- MEDLINE
DCOM- 19960808
LR  - 20220331
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 223
IP  - 2
DP  - 1996 Jun 14
TI  - Identification of a cDNA encoding a thiazide-sensitive sodium-chloride 
      cotransporter from the human and its mRNA expression in various tissues.
PG  - 324-8
AB  - We report here the identification of a cDNA encoding a human thiazide-sensitive 
      sodium-chloride cotransporter (hTSC) using a PCR-based method. The homology of 
      the hTSC with rat TSC (rTSC) and rat bumetanide-sensitive 
      sodium-potassium-chloride cotransporter (rBSC) was 86% and 64%, respectively, at 
      the nucleotide level, and 92% and 61%, respectively, at the amino acid level. 
      Using fluorescence in situ hybridization (FISH), the hTSC gene has been mapped to 
      chromosome 16q13. Northern blot analysis using polyA+RNA from various human 
      tissues, revealed a major 4.5 kb transcript and a minor 6.5 kb specifically in 
      the kidney, but low level of expression was also observed in small intestine, 
      placenta, prostate, colon, and spleen.
FAU - Chang, H
AU  - Chang H
AD  - Fourth Department of Internal Medicine, Faculty of Medicine, Universityof Tokyo, 
      Japan. hohang-tky@umin.u-tokyo.ac.jp
FAU - Tashiro, K
AU  - Tashiro K
FAU - Hirai, M
AU  - Hirai M
FAU - Ikeda, K
AU  - Ikeda K
FAU - Kurokawa, K
AU  - Kurokawa K
FAU - Fujita, T
AU  - Fujita T
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Benzothiadiazines)
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Complementary)
RN  - 0 (Diuretics)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Drug)
RN  - 0 (SLC12A3 protein, human)
RN  - 0 (Slc12a3 protein, rat)
RN  - 0 (Sodium Chloride Symporter Inhibitors)
RN  - 0 (Sodium Chloride Symporters)
RN  - 0 (Solute Carrier Family 12, Member 3)
RN  - 0 (Symporters)
RN  - 0 (thiazide receptor)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Benzothiadiazines
MH  - Carrier Proteins/*biosynthesis/*chemistry/genetics
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 16
MH  - Cloning, Molecular
MH  - Colon/metabolism
MH  - DNA Primers
MH  - DNA, Complementary
MH  - Diuretics
MH  - Female
MH  - Flounder
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Intestine, Small/metabolism
MH  - Kidney/metabolism
MH  - Male
MH  - Molecular Sequence Data
MH  - Organ Specificity
MH  - Placenta/metabolism
MH  - Polymerase Chain Reaction
MH  - Pregnancy
MH  - Prostate/metabolism
MH  - RNA, Messenger/biosynthesis
MH  - Rats
MH  - Receptors, Drug/*biosynthesis/*chemistry/genetics
MH  - Sequence Homology, Amino Acid
MH  - Sequence Homology, Nucleic Acid
MH  - Sodium Chloride Symporter Inhibitors/metabolism
MH  - Sodium Chloride Symporters
MH  - Solute Carrier Family 12, Member 3
MH  - Spleen/metabolism
MH  - *Symporters
MH  - *Transcription, Genetic
EDAT- 1996/06/14 00:00
MHDA- 1996/06/14 00:01
CRDT- 1996/06/14 00:00
PHST- 1996/06/14 00:00 [pubmed]
PHST- 1996/06/14 00:01 [medline]
PHST- 1996/06/14 00:00 [entrez]
AID - S0006-291X(96)90893-1 [pii]
AID - 10.1006/bbrc.1996.0893 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1996 Jun 14;223(2):324-8. doi: 
      10.1006/bbrc.1996.0893.