PMID- 8671330
OWN - NLM
STAT- MEDLINE
DCOM- 19961121
LR  - 20190512
IS  - 0268-1161 (Print)
IS  - 0268-1161 (Linking)
VI  - 11
IP  - 4
DP  - 1996 Apr
TI  - Localization and characterization of white blood cell populations within the 
      human ovary throughout the menstrual cycle and menopause.
PG  - 790-7
AB  - The purpose of this investigation was to localize and characterize white blood 
      cell populations in the human ovary through its physiological life cycle. Ovaries 
      from 30 women of reproductive age and from three post-menopausal women were 
      embedded in paraffin or frozen. Clinical information and pathology review were 
      used to obtain accurate menstrual cycle information and to ensure the absence of 
      ovarian disease. Tissue sections were stained for leukocyte phenotypes and the 
      numbers of white blood cells in the ovary were semiquantitatively assessed by two 
      separate examiners using a 0-3 plus (+) scoring system. Our results demonstrated 
      that macrophages and T lymphocytes were the primary immune cells of the ovary, 
      the concentrations of which were dependent on the location and stage of 
      development of the structures containing leukocytes. Developing follicles 
      contained few (+) macrophages located in the theca, while atretic follicles 
      possessed moderate (+2) numbers in the granulosa and few (+) to moderate (+2) 
      numbers in the theca. Newly formed corpora lutea contained few (+) macrophages, 
      while regressing corpora lutea contained abundant (+3) numbers. Human leukocyte 
      antigen (HLA)-DR positive cells were located predominantly at sites where 
      macrophages were present. T lymphocytes were generally not present in the 
      developing follicle but focal, small (+) numbers were observed in blood vessels 
      of the theca. Atretic follicles contained few (+) T lymphocytes in the granulosa 
      and few (+) to moderate (+2) numbers in the theca. Few (+) T lymphocytes were 
      present in new corpora lutea, while moderate (+2) to abundant (+3) numbers were 
      present in regressing corpora lutea. T lymphocytes at all sites were UCHL1 
      positive. The CD4 (T helper) to CD8 (T suppressor) ration in the corpus luteum 
      was 1:1. B-lymphocytes and natural killer cells were generally absent in the 
      pre-menopausal ovary. The post-menopausal ovary, in contrast, only contained few 
      (+) macrophages, T lymphocytes and natural killer cells in the stroma. In 
      conclusion, our results indicate that the human ovary is an immunologically 
      dynamic tissue containing activated macrophages and T lymphocytes which provide 
      an anatomical basis for immunoendocrine interactions within the ovary.
FAU - Best, C L
AU  - Best CL
AD  - Fearing Research Laboratory, Department of Obstetrics, Gynaecology, and 
      Reproductive Biology, Brigham and Women's Hospital, Harvard Medical School, 
      Boston, Massachusetts, USA.
FAU - Pudney, J
AU  - Pudney J
FAU - Welch, W R
AU  - Welch WR
FAU - Burger, N
AU  - Burger N
FAU - Hill, J A
AU  - Hill JA
LA  - eng
GR  - HD00815/HD/NICHD NIH HHS/United States
GR  - HD23547/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Hum Reprod
JT  - Human reproduction (Oxford, England)
JID - 8701199
SB  - IM
MH  - Adult
MH  - Aged
MH  - Cell Count
MH  - Female
MH  - Humans
MH  - Leukocytes/cytology
MH  - Macrophages/cytology
MH  - *Menopause/blood
MH  - *Menstrual Cycle/blood
MH  - Middle Aged
MH  - Ovary/*cytology
MH  - Postmenopause/blood
MH  - T-Lymphocytes/cytology
EDAT- 1996/04/01 00:00
MHDA- 1996/04/01 00:01
CRDT- 1996/04/01 00:00
PHST- 1996/04/01 00:00 [pubmed]
PHST- 1996/04/01 00:01 [medline]
PHST- 1996/04/01 00:00 [entrez]
AID - 10.1093/oxfordjournals.humrep.a019256 [doi]
PST - ppublish
SO  - Hum Reprod. 1996 Apr;11(4):790-7. doi: 10.1093/oxfordjournals.humrep.a019256.