PMID- 8671750
OWN - NLM
STAT- MEDLINE
DCOM- 19961107
LR  - 20190512
IS  - 0267-8357 (Print)
IS  - 0267-8357 (Linking)
VI  - 11
IP  - 3
DP  - 1996 May
TI  - Detection of the centromere in micronuclei by fluorescence in situ hybridization: 
      its application to the human lymphocyte micronucleus assay after treatment with 
      four suspected aneugens.
PG  - 285-90
AB  - The human lymphocyte micronucleus (MN) test combined with fluorescence in situ 
      hybridization (FISH) of a centromeric probe is considered a useful screening 
      assay to distinguish between clastogenic and aneugenic agents. Four suspected 
      aneuploidy-inducing chemicals, acetaldehyde (AA), diethylstilbestrol (DES), 
      diethylstilbestrol dipropionate (DESdp) and griseofulvin (GF), have been 
      evaluated with the assay. All compounds induced a significant increase of MN at 
      all doses tested. After the application of the FISH technique with a 
      pancentromeric DNA sequence, DES, DESdp and GF showed a statistically significant 
      increase in the percentage of positive signals compared with the control culture. 
      GF induced the highest percentage of centromere-positive MN observed to date 
      (>90% on average). AA did not show a significant difference in the percentage of 
      centromere-positive MN. The results indicate that in human lymphocytes DES, DESdp 
      and GF act primarily as aneugens, while AA seems capable of causing both 
      chromosome breakage and aneuploidy.
FAU - Migliore, L
AU  - Migliore L
AD  - Dipartimento di Scienze dell'Ambiente e del Territorio, Universita di Pisa, Via S 
      Giuseppe 22, 56100 Pisa, Italy.
FAU - Cocchi, L
AU  - Cocchi L
FAU - Scarpato, R
AU  - Scarpato R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Mutagenesis
JT  - Mutagenesis
JID - 8707812
RN  - 0 (Mutagens)
RN  - 32HRV3E3D5 (Griseofulvin)
RN  - 731DCA35BT (Diethylstilbestrol)
RN  - GO1N1ZPR3B (Acetaldehyde)
RN  - Y98CK3J0OL (diethylstilbestrol dipropionate)
SB  - IM
MH  - Acetaldehyde/toxicity
MH  - Aneuploidy
MH  - Centromere/*genetics
MH  - Diethylstilbestrol/analogs & derivatives/toxicity
MH  - Drug Evaluation, Preclinical/methods
MH  - Griseofulvin/toxicity
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Lymphocytes/*drug effects
MH  - Male
MH  - Micronuclei, Chromosome-Defective/*ultrastructure
MH  - *Micronucleus Tests
MH  - Mutagens/*toxicity
EDAT- 1996/05/01 00:00
MHDA- 1996/05/01 00:01
CRDT- 1996/05/01 00:00
PHST- 1996/05/01 00:00 [pubmed]
PHST- 1996/05/01 00:01 [medline]
PHST- 1996/05/01 00:00 [entrez]
AID - 10.1093/mutage/11.3.285 [doi]
PST - ppublish
SO  - Mutagenesis. 1996 May;11(3):285-90. doi: 10.1093/mutage/11.3.285.