PMID- 8671754
OWN - NLM
STAT- MEDLINE
DCOM- 19961008
LR  - 20190512
IS  - 0267-8357 (Print)
IS  - 0267-8357 (Linking)
VI  - 11
IP  - 4
DP  - 1996 Jul
TI  - Mitotic non-disjunction as a mechanism for in vitro aneuploidy induction by 
      X-rays in primary human cells.
PG  - 307-13
AB  - A collaborative study of three laboratories compared the induction of aneuploidy 
      by X-rays in human lymphocytes and fibroblasts. The induction of non-disjunction 
      versus chromosome loss by X-rays was investigated using a variety of aneuploidy 
      detection methods. Chromosome loss was determined by fluorescence in situ 
      hybridization (FISH) with pan-centromeric probes in cytochalasin-B-blocked 
      binucleated cells. Chromosome non-disjunction was estimated by FISH with 
      chromosome-specific centromeric probes in binucleated interphase cells. 
      Chromosomes were counted in parallel in lymphocyte metaphase cells; chromosome 
      counts of the whole karyotype and counts of chromosomes 2 and 8 using chromosome 
      paints. A major observation in spontaneous non-disjunction frequencies concerned 
      the clear difference in frequencies observed between the two painted chromosomes 
      in the same primary cells. When cells were irradiated elevated frequencies were 
      observed for all the different cytogenetic endpoints. Although only a small 
      number of the micronuclei were positive for the centromeric signal and presumably 
      contained whole chromosomes, the absolute number %oC+ increased with dose. Higher 
      rates of non-disjunction were found for irradiated cells; in fibroblasts a 
      statistically significant increase was observed at a dose of 0.5 Gy. The 
      detection of hyperdiploidy by means of chromosome counts and chromosome painting 
      revealed an increase from doses of 1 Gy and higher. Comparison of the different 
      methods detecting different endpoints indicates that non-disjunction may be an 
      important mechanism leading to spontaneous and X-ray-induced aneuploidy. The 
      relative radiosensitivity of aneuploidy induction was compared in two types of 
      primary human cells - lymphocytes and fibroblasts. For chromosome loss both cell 
      types showed similar results, whereas for non-disjunction fibroblasts seemed to 
      be more sensitive. However, these differences may reflect a different sensitivity 
      in the scoring methods used.
FAU - Kirsch-Volders, M
AU  - Kirsch-Volders M
AD  - Laboratory for Anthropogenetics, Vrije Universiteit Brussel, Pleinlaan 2, 1050 
      Brussels, Belgium.
FAU - Tallon, I
AU  - Tallon I
FAU - Tanzarella, C
AU  - Tanzarella C
FAU - Sgura, A
AU  - Sgura A
FAU - Hermine, T
AU  - Hermine T
FAU - Parry, E M
AU  - Parry EM
FAU - Parry, J M
AU  - Parry JM
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Mutagenesis
JT  - Mutagenesis
JID - 8707812
RN  - 3CHI920QS7 (Cytochalasin B)
SB  - IM
MH  - *Aneuploidy
MH  - Cells, Cultured
MH  - Cytochalasin B
MH  - Fibroblasts
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphocytes
MH  - Mitosis/*genetics/*radiation effects
MH  - Radiation Tolerance
EDAT- 1996/07/01 00:00
MHDA- 1996/07/01 00:01
CRDT- 1996/07/01 00:00
PHST- 1996/07/01 00:00 [pubmed]
PHST- 1996/07/01 00:01 [medline]
PHST- 1996/07/01 00:00 [entrez]
AID - 10.1093/mutage/11.4.307 [doi]
PST - ppublish
SO  - Mutagenesis. 1996 Jul;11(4):307-13. doi: 10.1093/mutage/11.4.307.