PMID- 8673535
OWN - NLM
STAT- MEDLINE
DCOM- 19960813
LR  - 20191210
IS  - 1077-9450 (Print)
IS  - 1077-9450 (Linking)
VI  - 12
IP  - 3
DP  - 1996 Jul
TI  - Sequence variation and subtyping of human and simian T-cell lymphotropic virus 
      type I strains from South Africa.
PG  - 298-302
AB  - We report on the subtyping of South African primate T-cell lymphotropic virus 
      type I (PTLV-I) strains by investigating the LTR region using sequence analysis 
      and restriction fragment length polymorphism (RFLP) techniques. DNA from either 
      uncultured peripheral blood mononuclear cells (PBMCs); cultured PBMC or cell 
      lines of eight human T-cell lymphotropic virus type I (HTLV-I); and two simian 
      T-cell lymphotropic virus type I (STLV-I) strains (Cercopithecus aethiops 
      pygerythrus) were amplified by polymerase chain reaction (PCR), cloned, and 
      sequenced. The samples originated from different geographical regions in South 
      Africa. Phylogenetic relationships were estimated using the neighbor-joining 
      method. The South African HTLV-I strains were of Cosmopolitan origin and similar 
      to each other. RFLP analysis confirmed this subtyping. A divergence of 0.3 to 
      1.6% between the Cosmopolitan strains was observed, while the divergence between 
      the HTLV-I and STLV-I strains ranged from 6.3 to 7%. The STLV-I strains were 
      closely related to that of a chimpanzee, providing evidence of interspecies 
      transmission.
FAU - Englebrecht, S
AU  - Englebrecht S
AD  - Department of Medical Virology, University of Stellenbosch and Tygerberg 
      Hospital, Tygerberg, South Africa.
FAU - van Rensburg, E J
AU  - van Rensburg EJ
FAU - Robson, B A
AU  - Robson BA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Acquir Immune Defic Syndr Hum Retrovirol
JT  - Journal of acquired immune deficiency syndromes and human retrovirology : 
      official publication of the International Retrovirology Association
JID - 9501482
SB  - IM
MH  - Animals
MH  - Chlorocebus aethiops
MH  - Deltaretrovirus Infections/veterinary/virology
MH  - Genetic Variation/*genetics
MH  - HTLV-I Infections/virology
MH  - Human T-lymphotropic virus 1/classification/*genetics
MH  - Humans
MH  - *Phylogeny
MH  - Polymorphism, Restriction Fragment Length
MH  - Repetitive Sequences, Nucleic Acid/*genetics
MH  - Sequence Analysis, DNA
MH  - Simian T-lymphotropic virus 1/classification/*genetics
MH  - South Africa
EDAT- 1996/07/01 00:00
MHDA- 1996/07/01 00:01
CRDT- 1996/07/01 00:00
PHST- 1996/07/01 00:00 [pubmed]
PHST- 1996/07/01 00:01 [medline]
PHST- 1996/07/01 00:00 [entrez]
AID - 10.1097/00042560-199607000-00011 [doi]
PST - ppublish
SO  - J Acquir Immune Defic Syndr Hum Retrovirol. 1996 Jul;12(3):298-302. doi: 
      10.1097/00042560-199607000-00011.