PMID- 8674502
OWN - NLM
STAT- MEDLINE
DCOM- 19960814
LR  - 20190512
IS  - 0141-5387 (Print)
IS  - 0141-5387 (Linking)
VI  - 18
IP  - 2
DP  - 1996 Apr
TI  - Stimulation of bone formation in the expanding mid-palatal suture by transforming 
      growth factor-beta 1 in the rat.
PG  - 169-79
AB  - The most intense endogenous transforming growth factor (TGF)-beta 1 expression 
      was detected in osteoblasts and fibroblasts in the mid-palatal suture 24 hours 
      after the start of palatal expansion with an immunohistochemical technique. Based 
      on these data, local effects of transforming growth factor-beta 1 (TGF-beta 1) in 
      the expanding mid-palatal suture of the rat were examined. Single doses of human 
      recombinant (rhu) TGF-beta 1 (40 and 200 ng, and 1 microgram) were injected into 
      the expanding mid-palatal suture 24 hours after expansion started. Calcein was 
      also injected immediately, and 6 and 12 days after expansion. The width of each 
      calcein label was measured to evaluate bone formation along the suture with bone 
      histomorphometry. In the experimental groups, bone formation during the 12-day 
      expansion period was stimulated significantly (P < 0.05), in a dose-dependent 
      manner, compared with that in the control group. Furthermore, bone formation 
      during the later stage (6-12 days) of the 12-day expansion period, was still 
      significantly higher than that in the control group. In addition, the amount of 
      bone formation in response to a course of 3 injections of 200 ng rhu TGF-beta 1 
      on days 3, 6, and 9 was almost equal to that induced by a single injection of 200 
      ng rhu TGF-beta 1 on day 1. These results suggest that TGF-beta 1 may play an 
      important role in bone formation at the active site of the suture in response to 
      rapid palatal expansion and application of TGF-beta 1 during the early stages may 
      induce rapid bone formation.
FAU - Sawada, M
AU  - Sawada M
AD  - Department of Orthodontics, Nihon University School of Dentistry, Chiba, Japan.
FAU - Shimizu, N
AU  - Shimizu N
LA  - eng
PT  - Journal Article
PL  - England
TA  - Eur J Orthod
JT  - European journal of orthodontics
JID - 7909010
RN  - 0 (Fluoresceins)
RN  - 0 (Indicators and Reagents)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Transforming Growth Factor beta)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
RN  - EC 3.1.3.2 (Acid Phosphatase)
RN  - V0YM2B16TS (fluorexon)
SB  - IM
MH  - Acid Phosphatase/analysis
MH  - Alkaline Phosphatase/analysis
MH  - Animals
MH  - Cranial Sutures/*drug effects/metabolism/physiology
MH  - Dose-Response Relationship, Drug
MH  - Fibroblasts/drug effects/metabolism
MH  - Fluoresceins
MH  - Indicators and Reagents
MH  - Male
MH  - Osteoblasts/drug effects/metabolism
MH  - Osteogenesis/*drug effects
MH  - *Palatal Expansion Technique
MH  - Palate/*drug effects/metabolism/physiology
MH  - Rats
MH  - Rats, Wistar
MH  - Recombinant Proteins
MH  - Transforming Growth Factor beta/administration & dosage/analysis/*pharmacology
EDAT- 1996/04/01 00:00
MHDA- 1996/04/01 00:01
CRDT- 1996/04/01 00:00
PHST- 1996/04/01 00:00 [pubmed]
PHST- 1996/04/01 00:01 [medline]
PHST- 1996/04/01 00:00 [entrez]
AID - 10.1093/ejo/18.2.169 [doi]
PST - ppublish
SO  - Eur J Orthod. 1996 Apr;18(2):169-79. doi: 10.1093/ejo/18.2.169.