PMID- 8676861
OWN - NLM
STAT- MEDLINE
DCOM- 19960815
LR  - 20190725
IS  - 0026-8925 (Print)
IS  - 0026-8925 (Linking)
VI  - 250
IP  - 5
DP  - 1996 Mar 20
TI  - Cis-elements important for the expression of the ADP-glucose pyrophosphorylase 
      small-subunit are located both upstream and downstream from its structural gene.
PG  - 581-92
AB  - ADP-glucose pyrophosphorylase (AGP) is a key regulatory enzyme in the 
      biosynthesis of starch in higher plants. Previous studies have suggested that, 
      unlike other plants that display tissue-specific AGP genes, potato expresses the 
      same AGP small-subunit gene (sAGP) in multiple tissues. This view was confirmed 
      by the spatial patterns of expression of the sAGP gene in transgenic potato 
      plants observed when a promoter-dependent-beta-glucuronidase (beta-GUS) system 
      was used. sAGP-beta-GUS chimeric gene fusions were expressed at high levels in 
      tubers and in many other starch-containing cells throughout the plant. Deletional 
      analysis of the 5'-upstream region of sAGP revealed that the observed spatial 
      patterns of expression were due to different regions of the promoter of sAGP 
      functioning in combination to confer cell- and organ-specific patterns of 
      expression. Depending on the tissue examined, the patterns of reporter-gene 
      expression were enhanced, suppressed, or altered when the 3'-nopaline-synthase 
      terminator was replaced by the 3'-flanking sequence of sAGP. The observed 
      cellular expression patterns of sAGP only partially overlap with the reported 
      expression patterns of the major large-subunit gene (lAGP) in leaves. Since AGP 
      is a heterotetrameric enzyme, composed of two sAGP and two lAGP subunits, this 
      difference in the cellular expression patterns as well as quantitative 
      differences in expression of the two AGP genes may account for the observed 
      post-transcriptional regulation, i.e., relatively high levels of transcript but 
      low levels of sAGP subunit in leaves.
FAU - Nakata, P A
AU  - Nakata PA
AD  - Department of Biochemistry/Biophysics, Washington State University, Pullman, 
      Washington 99164-6340, USA.
FAU - Okita, T W
AU  - Okita TW
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Germany
TA  - Mol Gen Genet
JT  - Molecular & general genetics : MGG
JID - 0125036
RN  - 0 (DNA Primers)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Recombinant Fusion Proteins)
RN  - EC 2.7.7.- (Nucleotidyltransferases)
RN  - EC 2.7.7.27 (Glucose-1-Phosphate Adenylyltransferase)
RN  - EC 3.2.1.31 (Glucuronidase)
SB  - IM
MH  - Agrobacterium tumefaciens
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - Consensus Sequence
MH  - DNA Primers
MH  - *Gene Expression Regulation, Enzymologic
MH  - Gene Expression Regulation, Plant
MH  - *Genes, Plant
MH  - Glucose-1-Phosphate Adenylyltransferase
MH  - Glucuronidase/biosynthesis
MH  - Macromolecular Substances
MH  - Molecular Sequence Data
MH  - Nucleotidyltransferases/*biosynthesis/genetics
MH  - Plant Leaves
MH  - Plant Roots
MH  - Plant Stems
MH  - Plants, Genetically Modified
MH  - Polymerase Chain Reaction
MH  - *Promoter Regions, Genetic
MH  - Recombinant Fusion Proteins/biosynthesis
MH  - *Regulatory Sequences, Nucleic Acid
MH  - Solanum tuberosum/*enzymology
EDAT- 1996/03/20 00:00
MHDA- 1996/03/20 00:01
CRDT- 1996/03/20 00:00
PHST- 1996/03/20 00:00 [pubmed]
PHST- 1996/03/20 00:01 [medline]
PHST- 1996/03/20 00:00 [entrez]
AID - 10.1007/BF02174446 [doi]
PST - ppublish
SO  - Mol Gen Genet. 1996 Mar 20;250(5):581-92. doi: 10.1007/BF02174446.