PMID- 8680698
OWN - NLM
STAT- MEDLINE
DCOM- 19960819
LR  - 20041117
IS  - 1073-449X (Print)
IS  - 1073-449X (Linking)
VI  - 154
IP  - 1
DP  - 1996 Jul
TI  - Shared amino acid motifs in T-cell receptor beta junctional regions of 
      bronchoalveolar T cells in patients with pulmonary sarcoidosis.
PG  - 50-6
AB  - To clarify the nature of the accumulated lung T cells of pulmonary sarcoidosis, 
      we studied bronchoalveolar and peripheral blood (PB) lymphocytes from Japanese 
      patients for expressed T-cell receptor beta-chain variable (TCR V beta) gene 
      usage. Quantitative polymerase chain reaction technique revealed that the V beta 
      repertoires of bronchoalveolar and PB T cells were heterogeneous. However, the 
      bronchoalveolar lymphocytes from five out of 10 patients showed 
      overrepresentation of T cells, with a few TCR V beta families, which varied among 
      individuals, possibly reflecting a difference in the human leukocyte antigen 
      (HLA) backgrounds. Nucleotide sequence analysis of third complementarity 
      determining region (CDR3) segments in overrepresented V beta families 
      demonstrated limited diversity of the transcripts. Two HLA-DR 12(5)+ individuals 
      with overrepresented V beta 6 gene transcripts in the lungs shared a common amino 
      acid motif within their CDR3. By contrast, non-overrepresented TCR V beta 
      transcripts did not show clonal expansion, and the oligoclonal clones in the lung 
      were not found in PB of the corresponding patient. These results showed that, at 
      least in subsets of Japanese patients with pulmonary sarcoidosis, a limited 
      number of T-cell clones are accumulated in the lung in response to a specific 
      antigen. They may mediate an immune response to shape pathology of the disease.
FAU - Usui, Y
AU  - Usui Y
AD  - Second Department of Internal Medicine, Tokyo Medical and Dental University, 
      Japan.
FAU - Kohsaka, H
AU  - Kohsaka H
FAU - Eishi, Y
AU  - Eishi Y
FAU - Saito, I
AU  - Saito I
FAU - Marumo, F
AU  - Marumo F
FAU - Miyasaka, N
AU  - Miyasaka N
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am J Respir Crit Care Med
JT  - American journal of respiratory and critical care medicine
JID - 9421642
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Receptors, Antigen, T-Cell, alpha-beta)
SB  - IM
MH  - Adult
MH  - Amino Acid Sequence
MH  - Bronchoalveolar Lavage Fluid/*cytology
MH  - Female
MH  - Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor
MH  - HLA-DR Antigens/analysis
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - Receptors, Antigen, T-Cell, alpha-beta/*genetics
MH  - Sarcoidosis, Pulmonary/genetics/*immunology
MH  - T-Lymphocytes/immunology
EDAT- 1996/07/01 00:00
MHDA- 1996/07/01 00:01
CRDT- 1996/07/01 00:00
PHST- 1996/07/01 00:00 [pubmed]
PHST- 1996/07/01 00:01 [medline]
PHST- 1996/07/01 00:00 [entrez]
AID - 10.1164/ajrccm.154.1.8680698 [doi]
PST - ppublish
SO  - Am J Respir Crit Care Med. 1996 Jul;154(1):50-6. doi: 
      10.1164/ajrccm.154.1.8680698.