PMID- 8681797
OWN - NLM
STAT- MEDLINE
DCOM- 19960822
LR  - 20220318
IS  - 0950-1991 (Print)
IS  - 0950-1991 (Linking)
VI  - 122
IP  - 7
DP  - 1996 Jul
TI  - Expression of the met receptor tyrosine kinase in muscle progenitor cells in 
      somites and limbs is absent in Splotch mice.
PG  - 2163-71
AB  - Hepatocyte growth factor/scatter factor (HGF/SF) stimulates proliferation, 
      dissociation, migration and morphogenesis of cells in culture. To investigate a 
      possible role for HGF/SF and its receptor, the Met tyrosine kinase, in embryonic 
      development, we have analyzed their expression in mouse embryos from day 7.5 of 
      gestation by whole-mount in situ hybridization. Met expression is first detected 
      in the ventral portion of somites at day 9.25 of gestation (22 somite embryo) at 
      the level of fore limb buds. As somites mature, met expression is detected in 
      caudal somites, and is confined to the lateral and media] tips of the 
      dermomyotome and dermomyotome/myotome respectively. In contrast, HGF/SF is 
      expressed exclusively in the mesodermal core of the limb bud. As the dermomyotome 
      elongates ventrolaterally, the met-expressing cells at the lateral tip appear to 
      detach from the somite, invade the limb bud and localize at the dorsal and 
      ventral limb sides in close proximity to HGF/SF-expressing cells. At later 
      stages, both met- and HGF/SF-expressing cells appear to migrate distally and 
      localize to the digit forming area of the developing hand plate. Met expression 
      in the lateral dermomyotome and limb bud coincides with expression of Pax-3, a 
      marker for migrating muscle precursor cells in the somite and limb. Splotch-2H 
      and Splotch-delayed mice, which harbor mutations in Pax-3, show major disruptions 
      in early limb muscle development. Significantly, no met-expressing cells were 
      observed in the limbs of homozygous Splotch-2H and Splotch-delayed animals, 
      whereas HGF/SF expression was not affected. The restricted expression of met to a 
      sub-population of Pax-3-expressing cells in the lateral tip of the dermomyotome, 
      demonstrates that met represents a unique molecular marker for this migratory 
      cell population. From these observations, together with the biological activities 
      of HGF/SF, we propose that in homozygous Splotch embryos the failure of muscle 
      precursors to migrate into and populate the limb bud results from a loss of met 
      expression in the cells at the ventrolateral edge of the somitic dermomyotome.
FAU - Yang, X M
AU  - Yang XM
AD  - Molecular Oncology Group, Royal Victoria Hospital, Montreal, Quebec, Canada.
FAU - Vogan, K
AU  - Vogan K
FAU - Gros, P
AU  - Gros P
FAU - Park, M
AU  - Park M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Development
JT  - Development (Cambridge, England)
JID - 8701744
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (PAX3 Transcription Factor)
RN  - 0 (Paired Box Transcription Factors)
RN  - 0 (RNA, Complementary)
RN  - 0 (Transcription Factors)
RN  - 138016-91-8 (Pax3 protein, mouse)
RN  - 63231-63-0 (RNA)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
RN  - EC 2.7.7.49 (RNA-Directed DNA Polymerase)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - DNA-Binding Proteins/analysis
MH  - Female
MH  - Forelimb/metabolism
MH  - Gene Expression Regulation, Developmental
MH  - Hepatocyte Growth Factor/*analysis/physiology
MH  - Hindlimb/metabolism
MH  - In Situ Hybridization
MH  - Limb Buds/cytology/*metabolism
MH  - Mesoderm/*metabolism
MH  - Mice
MH  - Mice, Neurologic Mutants
MH  - Molecular Sequence Data
MH  - Muscles/cytology/*embryology
MH  - PAX3 Transcription Factor
MH  - Paired Box Transcription Factors
MH  - Polymerase Chain Reaction
MH  - Pregnancy
MH  - RNA/isolation & purification
MH  - RNA, Complementary
MH  - RNA-Directed DNA Polymerase
MH  - Receptor Protein-Tyrosine Kinases/*analysis/physiology
MH  - Stem Cells/*metabolism
MH  - *Transcription Factors
EDAT- 1996/07/01 00:00
MHDA- 1996/07/01 00:01
CRDT- 1996/07/01 00:00
PHST- 1996/07/01 00:00 [pubmed]
PHST- 1996/07/01 00:01 [medline]
PHST- 1996/07/01 00:00 [entrez]
AID - 10.1242/dev.122.7.2163 [doi]
PST - ppublish
SO  - Development. 1996 Jul;122(7):2163-71. doi: 10.1242/dev.122.7.2163.