PMID- 8683100
OWN - NLM
STAT- MEDLINE
DCOM- 19960822
LR  - 20190512
IS  - 0022-1503 (Print)
IS  - 0022-1503 (Linking)
VI  - 87
IP  - 3
DP  - 1996 May-Jun
TI  - Derivation and characterization of a somatic cell hybrid containing the portion 
      of mouse chromosome 11 (MMU11) homologous to human chromosome 17q.
PG  - 252-8
AB  - To contribute to the physical gene map of mouse chromosome 11 (MMU11) and to 
      extend the mapping resources available for this chromosome, we have produced 
      mouse x rat somatic cell hybrids containing only bands B5 to E of MMU11. 
      Characterization of the hybrids by polymerase chain reaction (PCR) amplification 
      and Southern blot analyses of MMU11 markers revealed two hybrids, T16Ad14B and 
      T16Ad19A, that had selectively retained the 3(11) translocation product 
      containing distal MMU11 (bands B5-E). Cytogenetic analysis of the hybrid T16Ad14B 
      by fluorescence in situ hybridization (FISH) and conventional G-banding confirmed 
      the presence of the 3(11) translocation chromosome. Mapping of markers in both 
      the T16Ad14B and T16Ad19A hybrids localized the T16Ad translocation breakpoint 
      between the proximal markers Atplb2 and Acrb and the more distal markers Scya2 
      and Mpo. Loci for D11Mit5, Rpo2-1, Trp53, Glut4, Acrb, and Atplb2 could all be 
      localized proximal to the T16Ad breakpoint in band B5, between bands B1 and B5 on 
      MMU11.
FAU - Morris-Rosendahl, D J
AU  - Morris-Rosendahl DJ
AD  - Department of Zoology and Entomology, University of Pretoria, South Africa.
FAU - Schriever-Schwemmer, G
AU  - Schriever-Schwemmer G
FAU - Robinson, T J
AU  - Robinson TJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Hered
JT  - The Journal of heredity
JID - 0375373
SB  - IM
MH  - Animals
MH  - Blotting, Southern
MH  - Cell Line
MH  - *Chromosome Mapping
MH  - Chromosomes, Human, Pair 17
MH  - Fibroblasts/cytology
MH  - Humans
MH  - *Hybrid Cells
MH  - Mice/*genetics
MH  - Polymerase Chain Reaction
MH  - Rats
MH  - Translocation, Genetic
EDAT- 1996/05/01 00:00
MHDA- 1996/05/01 00:01
CRDT- 1996/05/01 00:00
PHST- 1996/05/01 00:00 [pubmed]
PHST- 1996/05/01 00:01 [medline]
PHST- 1996/05/01 00:00 [entrez]
AID - 10.1093/oxfordjournals.jhered.a022997 [doi]
PST - ppublish
SO  - J Hered. 1996 May-Jun;87(3):252-8. doi: 10.1093/oxfordjournals.jhered.a022997.