PMID- 8683215
OWN - NLM
STAT- MEDLINE
DCOM- 19960816
LR  - 20081121
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 77 ( Pt 6)
DP  - 1996 Jun
TI  - A nested set of six or seven subgenomic mRNAs is formed in cells infected with 
      different isolates of porcine reproductive and respiratory syndrome virus.
PG  - 1265-70
AB  - The subgenomic mRNAs (sg mRNA) of porcine reproductive and respiratory syndrome 
      virus (PRRSV) were characterized. The number of sg mRNAs, which form a 
      3'-coterminal nested set in PRRSV-infected cells, varied from six to seven among 
      PRRSV isolates with differing virulence. The additional species of sg mRNA in 
      some isolates of PRRSV was designated as sg mRNA 3-1. The leader-mRNA junction 
      sequences of sg mRNAs 3, 3-1 and 4 were found to contain a similar six nucleotide 
      sequence motif, U(G)UA(G/C)ACC. By comparing the 5'-terminal sequence of sg mRNA 
      3-1 with the genomic sequence of two isolates, ISU79 and ISU1894, it was found 
      that a point mutation, from U in isolate ISU1894 to C in isolate ISU79, led to 
      the acquisition of a new leader-mRNA junction sequence (UUGACC) in isolate ISU79, 
      and therefore an additional species of sg mRNA 3-1. A small ORF (3-1) was 
      identified at the 5' end of sg mRNA 3-1.
FAU - Meng, X J
AU  - Meng XJ
AD  - Department of Microbiology, Immunology and Preventive Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames 50011, USA.
FAU - Paul, P S
AU  - Paul PS
FAU - Morozov, I
AU  - Morozov I
FAU - Halbur, P G
AU  - Halbur PG
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (Codon)
RN  - 0 (DNA Primers)
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Arterivirus/*genetics/isolation & purification/metabolism
MH  - Arterivirus Infections/*veterinary/virology
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Cell Line
MH  - Codon
MH  - DNA Primers
MH  - DNA Probes
MH  - DNA, Complementary
MH  - Molecular Sequence Data
MH  - Open Reading Frames
MH  - RNA, Messenger/*biosynthesis
MH  - Sequence Homology, Amino Acid
MH  - Sequence Homology, Nucleic Acid
MH  - Swine
MH  - *Swine Diseases
MH  - Terminator Regions, Genetic
EDAT- 1996/06/01 00:00
MHDA- 1996/06/01 00:01
CRDT- 1996/06/01 00:00
PHST- 1996/06/01 00:00 [pubmed]
PHST- 1996/06/01 00:01 [medline]
PHST- 1996/06/01 00:00 [entrez]
AID - 10.1099/0022-1317-77-6-1265 [doi]
PST - ppublish
SO  - J Gen Virol. 1996 Jun;77 ( Pt 6):1265-70. doi: 10.1099/0022-1317-77-6-1265.