PMID- 8683390
OWN - NLM
STAT- MEDLINE
DCOM- 19960819
LR  - 20220316
IS  - 0022-3417 (Print)
IS  - 0022-3417 (Linking)
VI  - 178
IP  - 2
DP  - 1996 Feb
TI  - Differential in situ expression of the genes encoding the chemokines MCP-1 and 
      RANTES in human inflammatory bowel disease.
PG  - 201-6
AB  - Two chemotactic cytokines, monocyte chemoattractant protein-1 (MCP-1) and RANTES, 
      possibly contribute to the recruitment and activation of leukocytes in inflamed 
      tissues. The expression of these cytokine genes was evaluated in tissue sections 
      from resected bowel segments of 14 patients with inflammatory bowel disease (IBD) 
      and seven control patients by use of 35S-labelled antisense RNA probes. MCP-1 and 
      RANTES transcripts were generally increased in the intestinal mucosa of patients 
      with IBD, compared with controls. Whereas MCP-1 gene expression in the mucosa was 
      restricted to the lamina propria, the gene coding for RANTES was expressed in 
      intraepithelial lymphocytes and in the subepithelial lamina propria. Furthermore, 
      MCP-1 mRNA, but not RANTES mRNA, was abundant in vessel-associated cells, such as 
      endothelial cells, medial smooth muscle cells, and intraluminal cells; in smooth 
      muscle cells of the intestinal tunica muscularis; and in cells of the myenteric 
      plexus. Compared with controls, a significant increase of MCP-1-expressing cells 
      was observed in tissue specimens from patients with IBD, in endothelial cells of 
      venules, and in cells present in the lumen of intestinal vessels. Conversely, the 
      expression of MCP-1 mRNA in smooth muscle cells and myenteric plexus cells 
      appeared to be comparable in control and diseased intestines. The increased 
      number of MCP-1 and RANTES mRNA-expressing cells in mucosa from patients with IBD 
      suggests that these cytokines play a role in the pathogenesis of mucosal 
      inflammation. Furthermore, the expression of the MCP-1 gene in vessel-associated 
      cells may indicate its involvement in mechanisms regulating the adhesion of blood 
      monocytes to endothelial cells.
FAU - Mazzucchelli, L
AU  - Mazzucchelli L
AD  - Institute of Pathology, University of Bern, Switzerland.
FAU - Hauser, C
AU  - Hauser C
FAU - Zgraggen, K
AU  - Zgraggen K
FAU - Wagner, H E
AU  - Wagner HE
FAU - Hess, M W
AU  - Hess MW
FAU - Laissue, J A
AU  - Laissue JA
FAU - Mueller, C
AU  - Mueller C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Pathol
JT  - The Journal of pathology
JID - 0204634
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL5)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Adult
MH  - Case-Control Studies
MH  - Chemokine CCL2/genetics/*metabolism
MH  - Chemokine CCL5/genetics/*metabolism
MH  - Colitis, Ulcerative/*metabolism
MH  - Crohn Disease/*metabolism
MH  - Female
MH  - Gene Expression
MH  - Humans
MH  - In Situ Hybridization
MH  - Intestinal Mucosa/metabolism
MH  - Male
MH  - Middle Aged
MH  - RNA, Messenger/genetics
EDAT- 1996/02/01 00:00
MHDA- 2000/06/20 09:00
CRDT- 1996/02/01 00:00
PHST- 1996/02/01 00:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1996/02/01 00:00 [entrez]
AID - 10.1002/(SICI)1096-9896(199602)178:2<201::AID-PATH440>3.0.CO;2-4 [pii]
AID - 10.1002/(SICI)1096-9896(199602)178:2<201::AID-PATH440>3.0.CO;2-4 [doi]
PST - ppublish
SO  - J Pathol. 1996 Feb;178(2):201-6. doi: 
      10.1002/(SICI)1096-9896(199602)178:2<201::AID-PATH440>3.0.CO;2-4.