PMID- 8685681
OWN - NLM
STAT- MEDLINE
DCOM- 19960822
LR  - 20131121
IS  - 0036-7672 (Print)
IS  - 0036-7672 (Linking)
VI  - 126
IP  - 20
DP  - 1996 May 18
TI  - [Fluorescent in-situ hybridization technique (FISH) in the diagnosis of 
      Philadelphia translocation in chronic myeloid leukemia].
PG  - 855-63
AB  - The Philadelphia chromosome (Ph) resulting from translocation t(9;22)(q34;q11) is 
      observed in more than 90% of patients with chronic myeloid leukemia (CML). Its 
      molecular consequence is the genesis of a fusion gene BCR-ABL between the 5' 
      sequences of the BCR gene (chromosome 22) and the 3' end of the ABL gene 
      (chromosome 9). Fluorescence in situ hybridization (FISH) using specific DNA 
      probes provides a useful tool for the detection of t(9;22) and BCR-ABL 
      rearrangement. We report our results using the FISH technique for t(9;22) 
      assessment in the hematopoietic cells of patients with Ph-positive CML. The DNA 
      libraries pBS 9 and pBS 22 containing multiple sequences derived from chromosomes 
      9 and 22 have been used to identify t(9;22) in metaphase cells. The cos bcr-51 
      and cos abl-18 probes that hybridize to unique sequences specific to the BCR and 
      ABL genes have the ability to detect the BCR-ABL rearrangement in metaphase cells 
      as well as in interphase nuclei. FISH is a sensitive and specific technique that 
      represents a valuable complement to conventional cytogenetics. The BCR-ABL 
      rearrangement can be detected in metaphase spreads of insufficient quality or 
      from interphase nuclei in the case of terminally differentiated cells or of cells 
      which do not divide in vitro. When the efficiency of hybridization and detection 
      is good, a large number of cells can be analyzed. This is of major significance 
      in assessment of response to treatment and definition of a cytogenetic remission. 
      However, interphase cytogenetics may be difficult due to variations in signal 
      resolution and background level. The FISH technique can also be used to detect 
      the BCR-ABL rearrangement in cases of Ph negative BCR-ABL positive CML.
FAU - Martinet, D
AU  - Martinet D
AD  - Division autonome de genetique medicale, Centre hospitalier universitaire vaudois 
      (CHUV), Lausanne.
FAU - Muhlematter, D
AU  - Muhlematter D
FAU - Jotterand Bellomo, M
AU  - Jotterand Bellomo M
LA  - fre
PT  - English Abstract
PT  - Journal Article
PT  - Review
TT  - Application de la technique d'hybridation in situ fluorescente (FISH) au 
      diagnostic de la translocation de Philadelphie dans la leucemie myeloide 
      chronique.
PL  - Switzerland
TA  - Schweiz Med Wochenschr
JT  - Schweizerische medizinische Wochenschrift
JID - 0404401
RN  - 0 (DNA Probes)
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
SB  - IM
MH  - Color
MH  - DNA Probes
MH  - Fusion Proteins, bcr-abl/isolation & purification
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*genetics
MH  - *Philadelphia Chromosome
MH  - *Translocation, Genetic
RF  - 30
EDAT- 1996/05/18 00:00
MHDA- 1996/05/18 00:01
CRDT- 1996/05/18 00:00
PHST- 1996/05/18 00:00 [pubmed]
PHST- 1996/05/18 00:01 [medline]
PHST- 1996/05/18 00:00 [entrez]
PST - ppublish
SO  - Schweiz Med Wochenschr. 1996 May 18;126(20):855-63.