PMID- 8689673
OWN - NLM
STAT- MEDLINE
DCOM- 19960829
LR  - 20190920
IS  - 0143-4160 (Print)
IS  - 0143-4160 (Linking)
VI  - 19
IP  - 2
DP  - 1996 Feb
TI  - The lysosomal compartment as intracellular calcium store in MDCK cells: a 
      possible involvement in InsP3-mediated Ca2+ release.
PG  - 157-65
AB  - To test for a possible role of lysosomes in intracellular Ca2+ homeostasis, the 
      effects of glycyl-L-phenylalanine-beta-naphthylamide (GPN), known to permeabilize 
      these organelles by osmotic swelling, were studied in single MDCK cells. 
      Fluorescence of acridine orange, rhodol green dextran, lysotracker green and 
      FITC-dextran indicated that GPN (0.2 mmol/l) elicited a reversible 
      permeabilization of lysosomes. Cytosolic Ca2+ ([Ca2+]i) as determined by Fura-2 
      fluorescence increased from 60 +/- 11 to 534 +/- 66 nmol/l (n = 41) in the 
      presence of GPN. Whereas only a single intracellular Ca2+ release could be 
      induced by GPN in a Ca(2+)-free perfusate, repetitive release could be evoked in 
      Ca2+ containing solutions suggesting reuptake of Ca2+ into lysosomal stores. 
      GPN-induced Ca2+ release was blunted after pretreatment with thapsigargin (TG), 
      an inhibitor of Ca(2+)-ATPase, or repeated applications of ATP inducing Ca2+ 
      release from inositol trisphosphate (InsP3) sensitive Ca2+ stores. The effect of 
      ATP on Ca2+ release was, however, not abolished by preceding GPN treatment. 
      GPN-induced Ca2+ release from lysosomes was independent of InsP3 formation or 
      Ca(2+)-induced Ca2+ release, since it was unaffected by the phospholipase C 
      inhibitor U-73, 122 or by caffeine and ruthenium red. These results suggest that 
      Ca2+ largely accumulates in lysosomal vesicles. Moreover, these organelles seem 
      to be part or functionally coupled with InsP3-sensitive Ca2+ stores.
FAU - Haller, T
AU  - Haller T
AD  - Department of Physiology, University of Innsbruck, Austria.
FAU - Dietl, P
AU  - Dietl P
FAU - Deetjen, P
AU  - Deetjen P
FAU - Volkl, H
AU  - Volkl H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Cell Calcium
JT  - Cell calcium
JID - 8006226
RN  - 0 (Dipeptides)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Terpenes)
RN  - 21438-66-4 (glycylphenylalanine 2-naphthylamide)
RN  - 67526-95-8 (Thapsigargin)
RN  - 7664-41-7 (Ammonia)
RN  - 85166-31-0 (Inositol 1,4,5-Trisphosphate)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Adenosine Triphosphate/pharmacology
MH  - Ammonia/pharmacology
MH  - Animals
MH  - Calcium/*metabolism
MH  - Cell Compartmentation/drug effects/physiology
MH  - Cell Line
MH  - Cell Membrane Permeability/drug effects
MH  - Dipeptides/pharmacology
MH  - Dogs
MH  - Enzyme Inhibitors/pharmacology
MH  - Inositol 1,4,5-Trisphosphate/*metabolism
MH  - Kidney/cytology
MH  - Lysosomes/drug effects/*metabolism
MH  - Terpenes/pharmacology
MH  - Thapsigargin
EDAT- 1996/02/01 00:00
MHDA- 1996/02/01 00:01
CRDT- 1996/02/01 00:00
PHST- 1996/02/01 00:00 [pubmed]
PHST- 1996/02/01 00:01 [medline]
PHST- 1996/02/01 00:00 [entrez]
AID - S0143-4160(96)90084-6 [pii]
AID - 10.1016/s0143-4160(96)90084-6 [doi]
PST - ppublish
SO  - Cell Calcium. 1996 Feb;19(2):157-65. doi: 10.1016/s0143-4160(96)90084-6.