PMID- 8691141
OWN - NLM
STAT- MEDLINE
DCOM- 19960829
LR  - 20190508
IS  - 0022-1007 (Print)
IS  - 1540-9538 (Electronic)
IS  - 0022-1007 (Linking)
VI  - 184
IP  - 1
DP  - 1996 Jul 1
TI  - Humoral and cell-mediated autoimmunity in allergy to Aspergillus fumigatus.
PG  - 265-70
AB  - A cDNA encoding an allergenic protein was isolated from an Aspergillus fumigatus 
      (A. fumigatus) cDNA library displayed on the surface of filamentous phage. Serum 
      immunoglobulin E (IgE) from A. fumigatus-sensitized individuals was used to 
      enrich phage-expressing gene products binding to IgE. One of the cDNAs encoded a 
      26.7-kD protein that was identified as a manganese superoxide dismutase (MnSOD) 
      sharing 51.5% identity and 67.2% homology to the corresponding human enzyme. Both 
      human and A. fumigatus MnSOD coding sequences were expressed in Escherichia coli 
      as [His]6-tagged fusion proteins and purified by Ni(2+)-chelate affinity 
      chromatography. The two recombinant MnSODs were both recognized by IgE antibodies 
      from subjects allergic to the A. fumigatus MnSOD and elicited specific immediate 
      type allergic skin reactions in these individuals. Moreover, both human and A. 
      fumigatus MnSOD induced proliferation in peripheral blood mononuclear cells of A. 
      fumigatus-allergic subjects who showed specific IgE responses and reacted in skin 
      tests to MnSOD. These observations provide evidence for autoreactivity to the 
      human MnSOD in allergic persons sensitized to an environmental allergen from A. 
      fumigatus who share a high degree of sequence homology to the corresponding human 
      enzyme.
FAU - Crameri, R
AU  - Crameri R
AD  - Swiss Institute of Allergy and Asthma Research (SIAF), Davos, Switzerland.
FAU - Faith, A
AU  - Faith A
FAU - Hemmann, S
AU  - Hemmann S
FAU - Jaussi, R
AU  - Jaussi R
FAU - Ismail, C
AU  - Ismail C
FAU - Menz, G
AU  - Menz G
FAU - Blaser, K
AU  - Blaser K
LA  - eng
SI  - GENBANK/U53561
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (Antibodies, Fungal)
RN  - 0 (Antigens, Fungal)
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Complementary)
RN  - 0 (Fungal Proteins)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Antibodies, Fungal/*immunology
MH  - Antigens, Fungal/*immunology
MH  - Aspergillus fumigatus/*immunology
MH  - Autoimmunity/*immunology
MH  - Cloning, Molecular
MH  - DNA Primers/chemistry
MH  - DNA, Complementary
MH  - Fungal Proteins/genetics/immunology
MH  - Humans
MH  - Hypersensitivity/*immunology
MH  - Immunity, Cellular/*immunology
MH  - Molecular Sequence Data
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Skin Tests
MH  - Superoxide Dismutase/*immunology
PMC - PMC2192688
EDAT- 1996/07/01 00:00
MHDA- 1996/07/01 00:01
PMCR- 1997/01/01
CRDT- 1996/07/01 00:00
PHST- 1996/07/01 00:00 [pubmed]
PHST- 1996/07/01 00:01 [medline]
PHST- 1996/07/01 00:00 [entrez]
PHST- 1997/01/01 00:00 [pmc-release]
AID - 96305209 [pii]
AID - 10.1084/jem.184.1.265 [doi]
PST - ppublish
SO  - J Exp Med. 1996 Jul 1;184(1):265-70. doi: 10.1084/jem.184.1.265.