PMID- 8691319
OWN - NLM
STAT- MEDLINE
DCOM- 19960826
LR  - 20061115
IS  - 0022-3417 (Print)
IS  - 0022-3417 (Linking)
VI  - 178
IP  - 4
DP  - 1996 Apr
TI  - Interphase fluorescence in situ hybridization detection of t(2;13)(q35;q14) in 
      alveolar rhabdomyosarcoma--a diagnostic tool in minimally invasive biopsies.
PG  - 410-4
AB  - The identification of t(2;13)(q35;q14) is a useful aid in the accurate diagnosis 
      of rhabdomyosarcoma, distinguishing it from other small round cell tumours and 
      supporting the distinction between alveolar and embryonal forms. Cytogenetic 
      analysis is difficult and with the increased use of minimally invasive biopsy 
      methods and primary chemotherapy, adequate tumour material is not always 
      available. To overcome these difficulties, two-colour interphase fluorescence in 
      situ hybridization (FISH) to detect t(2;13)(q35;q14) was developed and its 
      utility in assessing minimally invasive biopsies was investigated. Two cosmid 
      clones which mapped proximal or distal to the breakpoint region 13q14 and one 
      yeast artificial chromosome clone that mapped distal to the 2q35 breakpoint were 
      identified. In interphase cells containing t(2;13)(q35;q14), the configuration of 
      cosmid and yeast artificial chromosome signals demonstrated the presence of the 
      translocation. Five cases of rhabdomyosarcoma were analysed by interphase FISH. 
      The t(2;13)(q35;q14) was detected in all four alveolar tumours and was confirmed 
      by cytogenetics in two cases, but was absent in one embryonal tumour. This 
      sensitive detection method is applicable to minimal amounts of fresh or frozen 
      tumour.
FAU - McManus, A P
AU  - McManus AP
AD  - Section of Paediatrics, Institute of Cancer Research, Sutton, Surrey, U.K.
FAU - O'Reilly, M A
AU  - O'Reilly MA
FAU - Jones, K P
AU  - Jones KP
FAU - Gusterson, B A
AU  - Gusterson BA
FAU - Mitchell, C D
AU  - Mitchell CD
FAU - Pinkerton, C R
AU  - Pinkerton CR
FAU - Shipley, J M
AU  - Shipley JM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Pathol
JT  - The Journal of pathology
JID - 0204634
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Biopsy/methods
MH  - Chromosomes, Human, Pair 13/*genetics
MH  - Chromosomes, Human, Pair 2/*genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Infant
MH  - Interphase/genetics
MH  - Male
MH  - Ploidies
MH  - Rhabdomyosarcoma, Alveolar/diagnosis/*genetics
MH  - *Translocation, Genetic
MH  - Tumor Cells, Cultured
EDAT- 1996/04/01 00:00
MHDA- 2000/06/20 09:00
CRDT- 1996/04/01 00:00
PHST- 1996/04/01 00:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1996/04/01 00:00 [entrez]
AID - 10.1002/(SICI)1096-9896(199604)178:4<410::AID-PATH508>3.0.CO;2-A [pii]
AID - 10.1002/(SICI)1096-9896(199604)178:4<410::AID-PATH508>3.0.CO;2-A [doi]
PST - ppublish
SO  - J Pathol. 1996 Apr;178(4):410-4. doi: 
      10.1002/(SICI)1096-9896(199604)178:4<410::AID-PATH508>3.0.CO;2-A.