PMID- 8691733
OWN - NLM
STAT- MEDLINE
DCOM- 19960829
LR  - 20190725
IS  - 0085-2538 (Print)
IS  - 0085-2538 (Linking)
VI  - 49
IP  - 4
DP  - 1996 Apr
TI  - Quantification of glomerular TGF-beta 1 mRNA in patients with diabetes mellitus.
PG  - 1120-6
AB  - Transforming growth factor-beta 1 (TGF-beta 1) is a primary determinant of the 
      mesangial expansion observed in diabetic nephropathy. In this study, we 
      quantitated the levels of intraglomerular TGF-beta 1 mRNA in patients with 
      diabetes mellitus using a competitive polymerase chain reaction (PCR) method. 
      Renal biopsy specimens were obtained from 29 patients with non-insulin-dependent 
      diabetes mellitus. Total RNA was extracted from the glomeruli and reverse 
      transcribed into cDNA with reverse transcriptase. To prepare samples containing 
      identical amounts of beta-actin cDNA (8 pg), we performed competitive PCR by 
      co-amplifying mutant templates of beta-actin with a unique EcoRI site. We also 
      used this competitive PCR method to measure TGF-beta 1 cDNA by co-amplifying 
      mutant templates of TGF-beta 1. We observed higher expression of TGF-beta 1 mRNA 
      in glomeruli of patients with diabetic nephropathy as compared with normal 
      glomeruli. Intraglomerular TGF-beta 1 mRNA was elevated, even in the early stage 
      of diabetic nephropathy. Moreover, levels of intraglomerular TGF-beta 1 mRNA 
      correlated with values of HbA1c. These data suggest that hyperglycemia induces 
      intraglomerular TGF-beta 1 mRNA expression in vivo, and that TGF-beta 1 
      overproduction may be associated with the progression of diabetic nephropathy.
FAU - Iwano, M
AU  - Iwano M
AD  - First Department of Internal Medicine, Nara Medical University, Japan.
FAU - Kubo, A
AU  - Kubo A
FAU - Nishino, T
AU  - Nishino T
FAU - Sato, H
AU  - Sato H
FAU - Nishioka, H
AU  - Nishioka H
FAU - Akai, Y
AU  - Akai Y
FAU - Kurioka, H
AU  - Kurioka H
FAU - Fujii, Y
AU  - Fujii Y
FAU - Kanauchi, M
AU  - Kanauchi M
FAU - Shiiki, H
AU  - Shiiki H
FAU - Dohi, K
AU  - Dohi K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Kidney Int
JT  - Kidney international
JID - 0323470
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor beta)
RN  - 9007-34-5 (Collagen)
SB  - IM
CIN - Kidney Int. 1998 Oct;54(4):1390-1. PMID: 9773681
MH  - Aged
MH  - Base Sequence
MH  - Biopsy
MH  - Blotting, Southern
MH  - Collagen/genetics
MH  - Diabetes Mellitus/*genetics/metabolism
MH  - Diabetic Neuropathies/*genetics/metabolism
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Kidney Glomerulus/*chemistry/pathology
MH  - Male
MH  - Microscopy, Confocal
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/analysis
MH  - Transforming Growth Factor beta/analysis/*genetics
EDAT- 1996/04/01 00:00
MHDA- 1996/04/01 00:01
CRDT- 1996/04/01 00:00
PHST- 1996/04/01 00:00 [pubmed]
PHST- 1996/04/01 00:01 [medline]
PHST- 1996/04/01 00:00 [entrez]
AID - S0085-2538(15)59456-8 [pii]
AID - 10.1038/ki.1996.162 [doi]
PST - ppublish
SO  - Kidney Int. 1996 Apr;49(4):1120-6. doi: 10.1038/ki.1996.162.