PMID- 8692206
OWN - NLM
STAT- MEDLINE
DCOM- 19960829
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 354
IP  - 1
DP  - 1996 Jul 5
TI  - Analysis of micronuclei induced by 1,3-butadiene and its metabolites using 
      fluorescence in situ hybridization.
PG  - 49-57
AB  - In our previous study, micronuclei (MN) were induced in bone marrow cells of mice 
      following inhalation exposure to 1300 ppm of 1,3-butadiene (BD) for 6 h per day 
      on 5 consecutive days, and in splenocytes of mice and rats treated 
      intraperitoneally with 80 mg/kg 1,2-epoxybutene (EB) and 30 mg/kg 
      1,2,3,4-diepoxybutane (DEB), respectively. In the present study, the nature of MN 
      induced by BD, EB and DEB was analyzed by means of fluorescence in situ 
      hybridization (FISH) using mouse minor satellite DNA and rat satellite I DNA as 
      probes. Percentages of MN with centromere signals (MN+) measured following 
      exposures to BD, EB and DEB indicate that these agents are predominantly 
      clastogens. Frequencies of MN+ per 1000 cells suggest that BD, EB and DEB are not 
      only strong clastogens, but also weak aneugens in mice. The weak aneugenic effect 
      of EB and DEB was not observed in rats. Analysis of the number of centromere 
      signals in individual MN, and the size distribution of MN with centromere signals 
      in EB- and DEB-treated animals, and in animals exposed to the positive controls 
      diethylstilbestrol (DES) and mitomycin C (MMC) led to the following conclusions: 
      (1) analysis of MN for the number of centromere signals may be a useful indicator 
      for identifying chemicals with aneugenic properties; (2) there is no correlation 
      between the size of MN and their origin (i.e., chromosome loss/gain or fragment).
FAU - Xiao, Y
AU  - Xiao Y
AD  - MGC-Department of Radiation Genetics and Chemical Mutagenesis, Leiden University, 
      Netherlands.
FAU - de Stoppelaar, J M
AU  - de Stoppelaar JM
FAU - Hoebee, B
AU  - Hoebee B
FAU - Schriever-Schwemmer, G
AU  - Schriever-Schwemmer G
FAU - Adler, I D
AU  - Adler ID
FAU - Tates, A D
AU  - Tates AD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Butadienes)
RN  - 0 (Mutagens)
RN  - JSD5FGP5VD (1,3-butadiene)
SB  - IM
MH  - Animals
MH  - Butadienes/metabolism/*toxicity
MH  - Centromere
MH  - *In Situ Hybridization, Fluorescence
MH  - Mice
MH  - Micronuclei, Chromosome-Defective/*drug effects
MH  - Mutagens/*toxicity
MH  - Rats
EDAT- 1996/07/05 00:00
MHDA- 1996/07/05 00:01
CRDT- 1996/07/05 00:00
PHST- 1996/07/05 00:00 [pubmed]
PHST- 1996/07/05 00:01 [medline]
PHST- 1996/07/05 00:00 [entrez]
AID - 0027510796000358 [pii]
AID - 10.1016/0027-5107(96)00035-8 [doi]
PST - ppublish
SO  - Mutat Res. 1996 Jul 5;354(1):49-57. doi: 10.1016/0027-5107(96)00035-8.