PMID- 8692965
OWN - NLM
STAT- MEDLINE
DCOM- 19960829
LR  - 20220318
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 93
IP  - 14
DP  - 1996 Jul 9
TI  - Direct binding of a soluble natural killer cell inhibitory receptor to a soluble 
      human leukocyte antigen-Cw4 class I major histocompatibility complex molecule.
PG  - 7178-83
AB  - Natural killer (NK) cells expressing specific p58 NK receptors are inhibited from 
      lysing target cells that express human leukocyte antigen (HLA)-C class I major 
      histocompatibility complex molecules. To investigate the interaction between p58 
      NK receptors and HLA-Cw4, the extracellular domain of the p58 NK receptor 
      specific for HLA-Cw4 was overexpressed in Escherichia coli and refolded from 
      purified inclusion bodies. The refolded NK receptor is a monomer in solution. It 
      interacts specifically with HLA-Cw4, blocking the binding of a p58-Ig fusion 
      protein to HLA-Cw4-expressing cells, but does not block the binding of a p58-Ig 
      fusion protein specific for HLA-Cw3 to HLA-Cw3-expressing cells. The bacterially 
      expressed extracellular domain of HLA-Cw4 heavy chain and beta2-microglobulin 
      were refolded in the presence of a HLA-Cw4-specific peptide. Direct binding 
      between the soluble p58 NK receptor and the soluble HLA-Cw4-peptide complex was 
      observed by native gel electrophoresis. Titration binding assays show that 
      soluble monomeric receptor forms a 1:1 complex with HLA-Cw4, independent of the 
      presence of Zn2+. The formation of complexes between soluble, recombinant 
      molecules indicates that HLA-Cw4 is sufficient for specific ligation by the NK 
      receptor and that neither glycoprotein requires carbohydrate for the interaction.
FAU - Fan, Q R
AU  - Fan QR
AD  - Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 
      02138, USA.
FAU - Garboczi, D N
AU  - Garboczi DN
FAU - Winter, C C
AU  - Winter CC
FAU - Wagtmann, N
AU  - Wagtmann N
FAU - Long, E O
AU  - Long EO
FAU - Wiley, D C
AU  - Wiley DC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (DNA Primers)
RN  - 0 (HLA-C Antigens)
RN  - 0 (HLA-C*04 antigen)
RN  - 0 (KIR2DL3 protein, human)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Protein Sorting Signals)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Receptors, KIR)
RN  - 0 (Receptors, KIR2DL3)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (beta 2-Microglobulin)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Chromatography, Gel
MH  - DNA Primers
MH  - HLA-C Antigens/*immunology/isolation & purification/metabolism
MH  - Humans
MH  - Inclusion Bodies/immunology
MH  - Killer Cells, Natural/*immunology
MH  - Macromolecular Substances
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - Protein Binding
MH  - Protein Sorting Signals/biosynthesis
MH  - Receptors, Immunologic/*immunology/isolation & purification/metabolism
MH  - Receptors, KIR
MH  - Receptors, KIR2DL3
MH  - Recombinant Fusion Proteins/immunology/isolation & purification/metabolism
MH  - beta 2-Microglobulin/biosynthesis/immunology
PMC - PMC38956
EDAT- 1996/07/09 00:00
MHDA- 1996/07/09 00:01
PMCR- 1997/01/09
CRDT- 1996/07/09 00:00
PHST- 1996/07/09 00:00 [pubmed]
PHST- 1996/07/09 00:01 [medline]
PHST- 1996/07/09 00:00 [entrez]
PHST- 1997/01/09 00:00 [pmc-release]
AID - 10.1073/pnas.93.14.7178 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1996 Jul 9;93(14):7178-83. doi: 
      10.1073/pnas.93.14.7178.