PMID- 8693064
OWN - NLM
STAT- MEDLINE
DCOM- 19960829
LR  - 20131121
IS  - 0033-7587 (Print)
IS  - 0033-7587 (Linking)
VI  - 146
IP  - 2
DP  - 1996 Aug
TI  - Ultrasoft 1.5 keV aluminum K X rays are efficient producers of complex chromosome 
      exchange aberrations as revealed by fluorescence in situ hybridization.
PG  - 144-50
AB  - The electron pairs generated by ultrasoft 1.5 keV aluminum K X-ray photons 
      deposit their energy in tracks of length < 70 nm and provide an ideal tool for 
      analyzing the spatial distribution of breaks and misrepair processes. We have 
      undertaken the analysis of changes in chromosome structure produced by aluminum K 
      X rays in untransformed HF12 human fibroblasts in G1 phase using fluorescence in 
      situ hybridization (FISH). Multicolored chromosome-specific DNA probes for 
      chromosomes 1 and 2 and an alpha-satellite pan-centromeric probe were used to 
      examine in vitro radiation-induced chromosome-type exchange aberrations. After 
      mean doses of 0.37-2.93 Gy the relative frequencies of complex exchanges, derived 
      from three or more breaks in two or more chromosomes, ranged from 15-35%. For the 
      classic break-age-and-rejoining theory to hold, very large interaction distances 
      are needed to account for this high frequency of multibreak interactions, unless 
      many sites pre-exist where several different chromosomes come very close 
      together. Alternatively, damaged DNA may be able to interact with adjacent 
      undamaged DNA, obviating the need for large rejoining distances.
FAU - Griffin, C S
AU  - Griffin CS
AD  - MRC Radiation and Genome Stability Unit, Harwell, Didcot, United Kingdom.
FAU - Stevens, D L
AU  - Stevens DL
FAU - Savage, J R
AU  - Savage JR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Radiat Res
JT  - Radiation research
JID - 0401245
RN  - CPD4NFA903 (Aluminum)
SB  - IM
MH  - Aluminum
MH  - Cell Survival/radiation effects
MH  - Cells, Cultured
MH  - *Chromosome Aberrations
MH  - Fibroblasts/radiation effects
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - X-Rays
EDAT- 1996/08/01 00:00
MHDA- 1996/08/01 00:01
CRDT- 1996/08/01 00:00
PHST- 1996/08/01 00:00 [pubmed]
PHST- 1996/08/01 00:01 [medline]
PHST- 1996/08/01 00:00 [entrez]
PST - ppublish
SO  - Radiat Res. 1996 Aug;146(2):144-50.