PMID- 8694800
OWN - NLM
STAT- MEDLINE
DCOM- 19960829
LR  - 20220330
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 224
IP  - 1
DP  - 1996 Jul 5
TI  - Identification of the snake venom substance that induces apoptosis.
PG  - 134-9
AB  - Hemorrhagic snake venom induces apoptosis of vascular endothelial cells [S. 
      Araki, T. Ishida, T. Yamamoto, K. Kaji, and H. Hayashi (1993) Biochem. Biophys. 
      Res. Comm. 190 , 148-153]. We have identified that a cytotoxic substance of 
      Korean snake venom which is responsible for the apoptosis is L-amino acid oxidase 
      (LAO). The purified enzyme is a homodimeric glycoprotein of 110,000 and is 
      capable of generating H2O2 by catalyzing oxidation of L-amino acid. In the 
      presence of the enzyme, cultured L1210 cell nuclei were splitted and showed the 
      characteristic ladder-like pattern of DNA fragmentation. The enzyme binds 
      directly to the cell surface, thereby increasing local concentration of H2O2. 
      However, experimental evidence suggests that the LAO-induced apoptotic mechanism 
      is distinguished from the one caused by exogenous H2O2.
FAU - Suhr, S M
AU  - Suhr SM
AD  - Department of Biochemistry, College of Science, Seoul, Korea.
FAU - Kim, D S
AU  - Kim DS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Agkistrodon venoms)
RN  - 0 (Crotalid Venoms)
RN  - 0 (DNA, Neoplasm)
RN  - 9007-49-2 (DNA)
RN  - EC 1.4.- (Amino Acid Oxidoreductases)
RN  - EC 1.4.3.2 (L-Amino Acid Oxidase)
SB  - IM
MH  - Amino Acid Oxidoreductases/isolation & purification/*toxicity
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Cell Line
MH  - Cell Membrane/ultrastructure
MH  - Crotalid Venoms/chemistry/*toxicity
MH  - DNA/drug effects
MH  - DNA, Neoplasm/drug effects
MH  - Electrophoresis, Polyacrylamide Gel
MH  - HL-60 Cells
MH  - HeLa Cells
MH  - Hematopoietic Stem Cells
MH  - Humans
MH  - L-Amino Acid Oxidase
MH  - Leukemia L1210
MH  - Leukemia, T-Cell
MH  - Lymphocytes/drug effects/ultrastructure
MH  - Mice
MH  - Spleen
MH  - Tumor Cells, Cultured
EDAT- 1996/07/05 00:00
MHDA- 1996/07/05 00:01
CRDT- 1996/07/05 00:00
PHST- 1996/07/05 00:00 [pubmed]
PHST- 1996/07/05 00:01 [medline]
PHST- 1996/07/05 00:00 [entrez]
AID - S0006-291X(96)90996-1 [pii]
AID - 10.1006/bbrc.1996.0996 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1996 Jul 5;224(1):134-9. doi: 10.1006/bbrc.1996.0996.