PMID- 8694821
OWN - NLM
STAT- MEDLINE
DCOM- 19960829
LR  - 20131121
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 224
IP  - 1
DP  - 1996 Jul 5
TI  - Transcriptional inhibition of the beta-amyloid precursor protein by 
      interferon-gamma.
PG  - 246-51
AB  - Attenuating beta-amyloid precursor protein (beta-APP) gene expression may have 
      relevance in diseases such as Alzheimer's disease, where beta-APP has been 
      implicated in neuropathological processes. We report here on the transcriptional 
      down-regulation of beta-APP by interferon-gamma (IFN-gamma) in SKNMC human 
      neuroblastoma cells. Treatment of the cells with IFN-gamma resulted in a 85% 
      dose-dependent inhibition of beta-APP promoter activity after 24 h of exposure, 
      with no changes observed at 5 h. For comparison, additional cytokines and 
      signaling agents were also investigated for effects on beta-APP promoter 
      activity. Elevated levels of activity were observed after treatment with phorbol 
      12-myristate 13-acetate and basic fibroblast growth factor whereas no significant 
      effects were seen after treatment with lipopolysaccharide or interleukin-1 beta. 
      Thus, IFN-gamma was shown here to be a suppressor of beta-APP promoter activity 
      and is the first cytokine reported to possess such down-regulating effects.
FAU - Ringheim, G E
AU  - Ringheim GE
AD  - Neuroscience Therapeutic Area, Hoechst Marion Roussel, Inc., Somerville, New 
      Jersey 08876, USA. ringhei1@brwhcc3.hcc.com
FAU - Szcepanik, A M
AU  - Szcepanik AM
FAU - Burgher, K L
AU  - Burgher KL
FAU - Petko, W
AU  - Petko W
FAU - Heroux, J A
AU  - Heroux JA
FAU - Cavalieri, F
AU  - Cavalieri F
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Amyloid beta-Protein Precursor)
RN  - 0 (Interleukin-1)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Recombinant Proteins)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - 82115-62-6 (Interferon-gamma)
RN  - EC 1.13.12.- (Luciferases)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Amyloid beta-Protein Precursor/*biosynthesis/*genetics
MH  - Cell Line
MH  - Cell Survival
MH  - Female
MH  - Fibroblast Growth Factor 2/pharmacology
MH  - Genomic Library
MH  - Humans
MH  - Interferon-gamma/*pharmacology
MH  - Interleukin-1/pharmacology
MH  - Kinetics
MH  - Lipopolysaccharides/pharmacology
MH  - Luciferases/biosynthesis
MH  - Placenta/metabolism
MH  - Pregnancy
MH  - Promoter Regions, Genetic
MH  - Recombinant Proteins/biosynthesis
MH  - Suppression, Genetic
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Transcription, Genetic/*drug effects
MH  - Transfection
EDAT- 1996/07/05 00:00
MHDA- 1996/07/05 00:01
CRDT- 1996/07/05 00:00
PHST- 1996/07/05 00:00 [pubmed]
PHST- 1996/07/05 00:01 [medline]
PHST- 1996/07/05 00:00 [entrez]
AID - S0006-291X(96)91015-3 [pii]
AID - 10.1006/bbrc.1996.1015 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1996 Jul 5;224(1):246-51. doi: 
      10.1006/bbrc.1996.1015.