PMID- 8696448
OWN - NLM
STAT- MEDLINE
DCOM- 19960904
LR  - 20191101
IS  - 0958-7578 (Print)
IS  - 0958-7578 (Linking)
VI  - 6
IP  - 1
DP  - 1996 Mar
TI  - Blood group A antigen expression in platelets is prominently associated with 
      glycoprotein Ib and IIb. Evidence for an A1/A2 difference.
PG  - 51-9
AB  - Blood group ABH antigens are associated with platelets as intrinsic determinants 
      and extrinsically adsorbed antigens, and exist both on glycosphingolipids and on 
      glycoproteins (GPs). We now provide direct evidence that the blood group ABH 
      antigens are prominently associated with platelet GPIb and GPIIb. By 
      immunoprecipitation, a murine monoclonal anti-A antibody precipitated 
      surface-biotin-labelled blood group A1 platelet membrane proteins with 
      electrophoretic characteristics identical to those of GPIb/IX and GPIIb/IIIa. By 
      immunoblotting of SDS-PAGE separated blood group A1 platelet proteins the 
      monoclonal anti-A antibody bound to proteins with electrophoretic characteristics 
      identical to those of GPIb and GPIIb. When immunoaffinity purified GPIb/IX and 
      GPIIb/IIIa, derived from blood group O, A1 and A2 platelets, were employed for 
      immunoblotting, GPIb and GPIIb only from A1 platelets bound the monoclonal anti-A 
      antibody. By ELISA, wherein monoclonal antibodies specific for GPIb (APl) and the 
      GPIIb/IIIa complex (AP2) were used to capture and hold antigens from platelet 
      lysate, human anti-A antibodies reacted with these proteins derived from blood 
      group A1 platelets; proteins from blood group A2, O and B platelets showed no 
      reactivity. These results indicate that blood group A antigen is associated with 
      GPIb and GPIIb derived from blood group A1 but not A2 platelets.
FAU - Hou, M
AU  - Hou M
AD  - Department of Medicine, Sahlgrenska University Hospital, University of Goteborg, 
      Sweden.
FAU - Stockelberg, D
AU  - Stockelberg D
FAU - Rydberg, L
AU  - Rydberg L
FAU - Kutti, J
AU  - Kutti J
FAU - Wadenvik, H
AU  - Wadenvik H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Transfus Med
JT  - Transfusion medicine (Oxford, England)
JID - 9301182
RN  - 0 (ABO Blood-Group System)
RN  - 0 (Antigens, Human Platelet)
RN  - 0 (Isoantigens)
RN  - 0 (Platelet Glycoprotein GPIIb-IIIa Complex)
RN  - 0 (Platelet Glycoprotein GPIb-IX Complex)
SB  - IM
MH  - ABO Blood-Group System/*immunology
MH  - *Antigens, Human Platelet
MH  - Blotting, Western
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Isoantigens/*blood
MH  - Platelet Glycoprotein GPIIb-IIIa Complex/*immunology
MH  - Platelet Glycoprotein GPIb-IX Complex/*immunology
MH  - Precipitin Tests
EDAT- 1996/03/01 00:00
MHDA- 1996/03/01 00:01
CRDT- 1996/03/01 00:00
PHST- 1996/03/01 00:00 [pubmed]
PHST- 1996/03/01 00:01 [medline]
PHST- 1996/03/01 00:00 [entrez]
AID - 10.1046/j.1365-3148.1996.d01-52.x [doi]
PST - ppublish
SO  - Transfus Med. 1996 Mar;6(1):51-9. doi: 10.1046/j.1365-3148.1996.d01-52.x.