PMID- 8697416
OWN - NLM
STAT- MEDLINE
DCOM- 19960830
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 89
IP  - 2
DP  - 1996 Jul 15
TI  - Detection of aneuploidy in interphase nuclei from non-small cell lung carcinomas 
      by fluorescence in situ hybridization using chromosome-specific repetitive DNA 
      probes.
PG  - 120-5
AB  - Interphase fluorescence in situ hybridization (FISH) is particularly useful for 
      detecting chromosome changes in tumors exhibiting a low mitotic index, as is the 
      case in many human non-small cell lung carcinomas (NSCLCs). A panel of 
      centromeric DNA probes specific for the autosomes 6, 7, 8, 9, 12, 17, and 18 was 
      used to analyze 17 primary NSCLCs. Evidence for aneuploidy was obtained in all 
      specimens. Gain of part or all of chromosome 7 was especially prominent, 
      occurring in a large population of cells in each of 14 tumors (82%). Extra 
      centromeric copies of chromosomes 6, 12, and 17 were also common, being observed 
      in 9 to 11 cases each. Gain of chromosome 9 was infrequent (three tumors). In two 
      cases, most of the nuclei had only a single chromosome 9 fluorescent signal. 
      Karyotypic findings were available for six cases and were generally consistent 
      with the FISH data. Both methods revealed considerable heterogeneity within 
      individual tumors. NSCLC specimens from 26 males were assayed with a Y-specific 
      centromeric sequence; loss of the Y was observed in 13 cases (50%). These 
      investigations demonstrate the feasibility of interphase FISH for the successful 
      analysis of numerical chromosome changes in NSCLCs.
FAU - Taguchi, T
AU  - Taguchi T
AD  - Department of Medical Oncology, Fox Chase Cancer Center, Philadelphia, 
      Pennsylvania 19111, USA.
FAU - Zhou, J Y
AU  - Zhou JY
FAU - Feder, M
AU  - Feder M
FAU - Litwin, S
AU  - Litwin S
FAU - Klein-Szanto, A J
AU  - Klein-Szanto AJ
FAU - Testa, J R
AU  - Testa JR
LA  - eng
GR  - CA-06927/CA/NCI NIH HHS/United States
GR  - CA-45745/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (DNA Probes)
SB  - IM
MH  - *Aneuploidy
MH  - Carcinoma, Non-Small-Cell Lung/*genetics
MH  - Cell Nucleus/ultrastructure
MH  - Chromosomes, Human, Pair 12
MH  - Chromosomes, Human, Pair 17
MH  - Chromosomes, Human, Pair 6
MH  - Chromosomes, Human, Pair 7
MH  - *DNA Probes
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Lung Neoplasms/*genetics
MH  - *Repetitive Sequences, Nucleic Acid
EDAT- 1996/07/15 00:00
MHDA- 1996/07/15 00:01
CRDT- 1996/07/15 00:00
PHST- 1996/07/15 00:00 [pubmed]
PHST- 1996/07/15 00:01 [medline]
PHST- 1996/07/15 00:00 [entrez]
AID - 0165-4608(95)00355-X [pii]
AID - 10.1016/0165-4608(95)00355-x [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1996 Jul 15;89(2):120-5. doi: 
      10.1016/0165-4608(95)00355-x.